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鸡胚总转移核糖核酸的分级分离及丙氨酸转移核糖核酸的纯化研究。

Studies on the fractionation of total transfer ribonucleic acid and purification of an alanine transfer ribonucleic acid from chick embryo.

作者信息

Anandaraj M P, Cherayil J D

出版信息

Biochem J. 1974 Sep;141(3):647-53. doi: 10.1042/bj1410647.

DOI:10.1042/bj1410647
PMID:4463955
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1168169/
Abstract

Chick embryo tRNA, prepared by a simple large-scale method, was fractionated on three different ion-exchange columns. In all cases simple chromatographic patterns for various tRNA species were observed, indicating the presence of only a few major species of tRNA for each amino acid. By repeated chromatography one species of alanine tRNA was purified to approx. 80% purity. T(1) ribonuclease digest of this purified tRNA gave a simple chromatographic pattern. Because of the simplicity of the method of preparation of tRNA from this readily available source and the presence of only a few species of tRNA for each amino acid, chick embryo is suited for the study of tRNA and its various functions in higher systems.

摘要

采用一种简单的大规模方法制备的鸡胚tRNA，在三种不同的离子交换柱上进行了分级分离。在所有情况下，均观察到各种tRNA种类的简单色谱图谱，表明每种氨基酸仅存在少数几种主要的tRNA种类。通过反复色谱法，一种丙氨酸tRNA被纯化至约80%的纯度。这种纯化的tRNA经T(1)核糖核酸酶消化后产生简单的色谱图谱。由于从这种容易获得的来源制备tRNA的方法简单，且每种氨基酸仅存在少数几种tRNA种类，鸡胚适合用于研究tRNA及其在高等系统中的各种功能。

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The separation of soluble ribonucleic acids on benzoylated diethylaminoethylcellulose.在苯甲酰化二乙氨基乙基纤维素上分离可溶性核糖核酸。
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Isolation of amino acyl oligonucleotides from T-1 ribonuclease digests of soluble ribonucleic acid charged with serine, glycine, threonine, and alanine.从用丝氨酸、甘氨酸、苏氨酸和丙氨酸负载的可溶性核糖核酸的T-1核糖核酸酶消化物中分离氨酰寡核苷酸。
Biochemistry. 1966 Apr;5(4):1323-32. doi: 10.1021/bi00868a028.
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