利用二维电泳技术鉴定和定位新的小鼠基因。
Use of two-dimensional electrophoresis to identify and map new mouse genes.
作者信息
Elliott R W
出版信息
Genetics. 1979 Feb;91(2):295-308. doi: 10.1093/genetics/91.2.295.
Abstract
Cytosol polypeptides from mouse liver have been examined using two-dimensional electrophoresis. About 250 spots were readily discernible. When cytosols from strains BALB/cBy and C57BL/6By were compared eight genetically determined differences were observed. Other strain pairs show comparable numbers of differences. These eight phenotypes were scored in seven recombinant inbred lines derived from the two parental strains, and their strain distribution patterns were compared with previously determined patterns for other genetic markers that differ between the two progenitor strains. Using this information, tentative chromosomes assignments for the genes controlling five of the variant phenotypes have been made, and two of the assignments have been confirmed using congenic resistant strains. These eight genes will be useful reference markers in future crosses designed to map new genes.
摘要
已使用二维电泳法对小鼠肝脏的胞质溶胶多肽进行了检测。大约250个斑点清晰可见。比较BALB/cBy和C57BL/6By品系的胞质溶胶时，观察到8个由基因决定的差异。其他品系对也显示出相当数量的差异。在源自两个亲本品系的7个重组近交系中对这8种表型进行了评分，并将它们的品系分布模式与先前确定的两个祖系品系之间不同的其他遗传标记的模式进行了比较。利用这些信息，已对控制5种变异表型的基因进行了初步的染色体定位，并且其中两项定位已通过同源抗性品系得到了证实。这8个基因将成为未来旨在绘制新基因图谱的杂交实验中的有用参考标记。
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