Masihi K N, Jíra J
Folia Parasitol (Praha). 1979;26(1):9-13.
Suitable procedures for separation of various stages of Toxoplasma gondii by density gradient centrifugation have been delineated using peritoneal exudate of infected mouse as a model. Separation from host cells was effected by gradient centrifugation at 450 g for 30 minutes. Using Ficoll, dextran and sucrose, average parasite recoveries by pooling up to and including the peak fraction of 73.65%, 66.18% and 65.68% respectively were obtained. Toxoplasma trophozoites peaked at density of 1.040 g/ml with Ficoll, 1.060 g/ml with dextran and 1.110 g/ml with sucrose. In view of successful separation of exo-enteric stages of Toxoplasma by density gradient centrifugation, possible application of this method to isolation of various endo-enteric stages is discussed.
已利用感染小鼠的腹腔渗出液作为模型，描绘了通过密度梯度离心法分离刚地弓形虫各个阶段的合适程序。通过在450g下进行30分钟的梯度离心，实现了从宿主细胞中分离。使用菲可、葡聚糖和蔗糖，通过合并直至并包括峰值部分，平均寄生虫回收率分别为73.65%、66.18%和65.68%。滋养体在菲可中的密度峰值为1.040g/ml，在葡聚糖中的密度峰值为1.060g/ml，在蔗糖中的密度峰值为1.110g/ml。鉴于通过密度梯度离心成功分离了弓形虫的肠外阶段，讨论了该方法在分离各种肠内阶段方面的可能应用。
