使用菲可-泛影葡胺不连续密度梯度一步从外周血中分离粒细胞。与右旋糖酐分离法的比较研究。

Separation of granulocytes from peripheral blood in a single step using discontinuous density gradients of Ficoll-Urografin. A comparative study with separation by dextran.

作者信息

Aguado M T, Pujol N, Rubiol E, Tura M, Celada A

出版信息

J Immunol Methods. 1980;32(1):41-50. doi: 10.1016/0022-1759(80)90115-5.

Abstract

A single-step method for separating granulocytes from peripheral blood using two different gradients of Ficoll-Urografin (F-U) density 1.075 and 1.097 g/ml) is described. The morphological, cytochemical and ultrastructural properties of neutrophils isolated by this method were compared to those isolated by dextran-enhanced sedimentation and neutrophils from the peripheral blood. These studies indicated fewer alterations in neutrophils separated by F-U centrifugation than in neutrophils concentrated by dextran. Finally, the granulocyte layer recovered by F-U fractionation was 98% pure (dextran 71%) and yielded 64% (dextran 79%) of total granulocytes.

摘要

本文描述了一种使用密度为1.075和1.097 g/ml的两种不同梯度的菲可-泛影葡胺（F-U）从外周血中分离粒细胞的单步方法。将通过该方法分离的中性粒细胞的形态学、细胞化学和超微结构特性与通过葡聚糖增强沉降法分离的中性粒细胞以及外周血中的中性粒细胞进行了比较。这些研究表明，通过F-U离心分离的中性粒细胞的变化比通过葡聚糖浓缩的中性粒细胞少。最后，通过F-U分级分离回收的粒细胞层纯度为98%（葡聚糖为71%），粒细胞总产量为64%（葡聚糖为79%）。