Robert R, Mauras G, Senet J M, Hocquet P
Biomed Pharmacother. 1982 Mar;36(2):98-100.
Purification procedures of Toxoplasma gondii suspensions are described. These methods were designed for the elimination of leucocytes or TG 180 cells. We have been using either gradient centrifugation in Ficoll or gel filtration. In the first case, only leucocytes are eliminated (95% of the initial content). On the other hand, gel filtration allowed the elimination of TG 180 cells. The yield (67% to 98%) depends on the chosen gel (Ultrogel AcA 202 or Sephadex G25 fine), and on the amount of TG 180 cells present before filtration.
本文描述了弓形虫悬液的纯化程序。这些方法旨在去除白细胞或TG 180细胞。我们一直在使用Ficoll梯度离心法或凝胶过滤法。在第一种情况下，仅去除白细胞（初始含量的95%）。另一方面，凝胶过滤法可去除TG 180细胞。产率（67%至98%）取决于所选的凝胶（Ultrogel AcA 202或Sephadex G25 fine）以及过滤前存在的TG 180细胞数量。
