一种用于联合检测人外周血淋巴细胞亚群的更简便方法。

A simpler method for combined detection of human peripheral lymphocyte subpopulations.

作者信息

Lee Y, Yoshizawa Y, Carr R G, Yokoyama M M

出版信息

Int Arch Allergy Appl Immunol. 1979;59(1):114-6. doi: 10.1159/000232247.

Abstract

A method for the simultaneous enumeration of human lymphocyte subpopulations was developed by mixing lymphocytes, sheep red blood cells and immunobeads. Thymus-dependent lymphocytes (T cells) are detected by rosette formation with AET-treated sheep blood cells, and thymus-independent lymphocytes (B cells) are identified by rosette formation with polyacrylamide gel coated with antibody specific to both light chains (chi and lambda) of immunoglobulin. The major advantage of this technique is that it is simple, convenient and reproducible. This method also detects lymphocytes possessing both T and B cell markers (double-marker lymphocytes). Three different types of rosettes are distinquishable under light-microscopic observation, and this method may be valuable in clinical use.

摘要

通过混合淋巴细胞、绵羊红细胞和免疫磁珠，开发出一种同时计数人淋巴细胞亚群的方法。通过与经AET处理的绵羊血细胞形成玫瑰花结来检测胸腺依赖性淋巴细胞（T细胞），并通过与涂有针对免疫球蛋白两条轻链（κ和λ）的特异性抗体的聚丙烯酰胺凝胶形成玫瑰花结来鉴定非胸腺依赖性淋巴细胞（B细胞）。该技术的主要优点是简单、方便且可重复。此方法还可检测同时具有T和B细胞标志物的淋巴细胞（双标志物淋巴细胞）。在光学显微镜观察下可区分三种不同类型的玫瑰花结，该方法在临床应用中可能具有重要价值。