通过介电方法对肌红蛋白溶液水合作用的研究。
An investigation by dielectric methods of hydration in myoglobin solutions.
作者信息
Grant E H, Mitton B G, South G P, Sheppard R J
出版信息
Biochem J. 1974 May;139(2):375-80. doi: 10.1042/bj1390375.
Abstract
The permittivities of three solutions of sperm-whale myoglobin of different concentrations were measured in the frequency range 300-1300MHz at 20 degrees C by using a coaxial-line technique. These results were combined with those measured previously at frequencies below 10MHz. Two methods are described for calculating the extent of macromolecular hydration from the data. The more reliable method yields results of approx. 0.25g of H(2)O/g of protein, which is in satisfactory agreement with the theoretically calculated value. Agreement with the value found from the rotational motion of the molecule is not so close, which is probably caused by the different meanings that may be ascribed to the term hydration.
摘要
在20摄氏度下，使用同轴线技术在300 - 1300MHz频率范围内测量了三种不同浓度的抹香鲸肌红蛋白溶液的电容率。这些结果与之前在低于10MHz频率下测量的结果相结合。描述了两种从数据计算大分子水合程度的方法。更可靠的方法得出的结果约为每克蛋白质0.25克H₂O，这与理论计算值吻合良好。与从分子旋转运动得出的值的吻合度没那么高，这可能是由于水合这个术语可能具有不同含义所致。
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