Parmley R T, Ostroy F, Gams R A, DeLucas L
J Histochem Cytochem. 1979 Feb;27(2):681-5. doi: 10.1177/27.2.448060.
To evaluate the ultrastructural distribution of transferrin on the surface of L1210 ascites tumor cells, we used ferrocyanide to stain ferric iron (Prussian blue reaction) in transferrin, as well as in ferritin conjugated to antibody that was immunologically attached to the transferrin. Small deposits averaging 5 nm in diameter identified transferrin iron, whereas large cuboidal deposits averaging 50 nm in diameter stained ferritin conjugated-antibody that was bound to both transferrin and apotransferrin on the cell surface. The ability of transferrin to deliver iron to ascites tumor cells was confirmed by kinetic studies of transferrin labeled with 59Fe and 125I. These preliminary results are consistent with release of transferrin iron at the cell surface and demonstrate additional uses for ferrocyanide in ultrastructural cytochemical techniques.
为了评估转铁蛋白在L1210腹水肿瘤细胞表面的超微结构分布，我们使用亚铁氰化物对转铁蛋白中的三价铁进行染色（普鲁士蓝反应），以及对与免疫附着在转铁蛋白上的抗体结合的铁蛋白进行染色。平均直径为5nm的小沉积物可识别转铁蛋白铁，而平均直径为50nm的大立方形沉积物则对与细胞表面转铁蛋白和脱铁转铁蛋白结合的铁蛋白结合抗体进行染色。用59Fe和125I标记的转铁蛋白的动力学研究证实了转铁蛋白向腹水肿瘤细胞输送铁的能力。这些初步结果与转铁蛋白铁在细胞表面的释放一致，并证明了亚铁氰化物在超微结构细胞化学技术中的其他用途。
