Sullivan A L, Grasso J A, Weintraub L R
Blood. 1976 Jan;47(1):133-43.
Electron-microscopic examination of rat reticulocytes and normoblasts incubated with transferrin conjugated to ferritin or ferritin-labeled antitransferrin revealed binding of ferritin conjugates to the surface membrane, and uptake of ferritin conjugates in micropinocytotic vesicles. No binding or endocytosis of ferritin was visualized when rat reticulocytes or normoblasts were incubated with ferritin alone or ferritin conjugated to nonspecific rabbit IgG. These observations support the concept that transferrin binds to a surface membrane receptor and is subsequently internalized by the developing red cell. Time course and temperature dependence studies suggest the endocytosis of transferrin may be an important mechanism in delivery of iron to the developing red cell.
用与铁蛋白偶联的转铁蛋白或铁蛋白标记的抗转铁蛋白孵育大鼠网织红细胞和正成红细胞后进行电子显微镜检查，结果显示铁蛋白偶联物与表面膜结合，并通过微胞饮小泡摄取铁蛋白偶联物。当大鼠网织红细胞或正成红细胞单独与铁蛋白或与非特异性兔IgG偶联的铁蛋白孵育时，未观察到铁蛋白的结合或内吞作用。这些观察结果支持了这样一种概念，即转铁蛋白与表面膜受体结合，随后被发育中的红细胞内化。时间进程和温度依赖性研究表明，转铁蛋白的内吞作用可能是铁传递给发育中的红细胞的重要机制。
