Musson R A, Henson P M
J Immunol. 1979 May;122(5):2026-31.
Human and rabbit peripheral blood monocytes normally adhere to plastic tissue culture plates in vitro when they are suspended in Hanks' media. Increasing amounts of autologous serum or heat-inactivated plasma in the cell suspensions prevented the adherence of both monocytes and lymphocytes. The inhibitory effect of plasma was separated into three areas of activity by chromatography on Sephacryl S-200. The profile of inhibitory activity did not coincide with the protein elution profile, suggesting that inhibition was not a nonspecific protein effect. A layer of adherent platelets overcame the inhibitory effect of plasma on monocyte adherence. Platelets selectively increased monocyte as opposed to lymphocyte adherence and this was specific for platelets in that neither neutrophils nor fibroblasts could substitute for platelets. Both plasma and platelets acted directly on monocytes.
当人及兔的外周血单核细胞悬浮于汉克斯培养基中时，它们通常会在体外黏附于塑料组织培养板。细胞悬液中自体血清或热灭活血浆的量增加会阻止单核细胞和淋巴细胞的黏附。通过在Sephacryl S - 200上进行层析，血浆的抑制作用被分离为三个活性区域。抑制活性图谱与蛋白质洗脱图谱不一致，这表明抑制作用并非非特异性蛋白质效应。一层黏附的血小板可克服血浆对单核细胞黏附的抑制作用。血小板选择性地增加单核细胞而非淋巴细胞的黏附，并且这对血小板具有特异性，因为中性粒细胞和成纤维细胞都不能替代血小板。血浆和血小板均直接作用于单核细胞。
