源自人单核细胞培养的多核巨细胞的功能和生化研究。
Functional and biochemical studies of multinucleated giant cells derived from the culture of human monocytes.
作者信息
Schlesinger L, Musson R A, Johnston R B
出版信息
J Exp Med. 1984 Apr 1;159(4):1289-94. doi: 10.1084/jem.159.4.1289.
Abstract
We compared phagocytic and metabolic activities of multinucleated giant cells (MGC) and macrophages derived from human monocytes after 9-14 d in culture. Phagocytosis of sheep erythrocytes (E) coated with IgG, of E coated with IgM and complement, and of Candida albicans was comparable in MGC and macrophages. The same percentage of ingested fungi was killed by MGC (24 +/- 4%) and macrophages (21 +/- 5%). Approximately 70% of MGC and macrophages exhibited superoxide-dependent reduction of nitroblue tetrazolium during stimulation. Ia antigen was present on approximately 75% of both cell types. Analysis of cell populations separated by nuclear fluorescence indicated that beta-glucosaminidase, acid phosphatase, and beta-glucuronidase activity per cell was higher in MGC, but specific activity of these enzymes was greater in macrophages. These results suggest that MGC have the capacity to function like macrophages in host defense against infection.
摘要
我们比较了培养9 - 14天后源自人单核细胞的多核巨细胞（MGC）和巨噬细胞的吞噬及代谢活性。MGC和巨噬细胞对包被IgG的绵羊红细胞（E）、包被IgM和补体的E以及白色念珠菌的吞噬作用相当。MGC（24 +/- 4%）和巨噬细胞（21 +/- 5%）杀死的摄入真菌比例相同。在刺激过程中，约70%的MGC和巨噬细胞表现出超氧化物依赖性的硝基蓝四氮唑还原。两种细胞类型中约75%都存在Ia抗原。通过核荧光分离细胞群体的分析表明，MGC中每个细胞的β - 葡糖胺酶、酸性磷酸酶和β - 葡糖醛酸酶活性更高，但这些酶的比活性在巨噬细胞中更大。这些结果表明，MGC在宿主抗感染防御中具有类似巨噬细胞的功能能力。
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