坏血病豚鼠脑中的亚细胞抗坏血酸

Subcellular ascorbic acid in scorbutic guinea pig brain.

作者信息

Kuo C H, Yonehara N, Yoshida H

出版信息

J Nutr Sci Vitaminol (Tokyo). 1979;25(1):9-13. doi: 10.3177/jnsv.25.9.

DOI:10.3177/jnsv.25.9

Abstract

In scorbutic guinea pigs fed an ascorbic acid-deficient diet for 18 days, the ascorbic acid contents of the liver and plasma were less than 4% those of the control animals, while the content in the brain was about one-third that of the controls. In normal animals, the concentration of ascrobic acid was highest (7.55 +/- 1.46 micrograms of ascorbic acid/mg protein) in the S3 fraction and was also fairly high (5.37 +/- 0.91) in the P2p fraction (cytoplasm of nerve terminals). During ascorbic acid deficiency, the contents in the P2p and microsome fractions decreased slightly faster than those in other fractions. Even after 18 days' deficiency, no significant change was found in the acetylcholine content of the brain.

摘要

在喂食缺乏抗坏血酸饮食18天的坏血病豚鼠中，肝脏和血浆中的抗坏血酸含量不到对照动物的4%，而大脑中的含量约为对照动物的三分之一。在正常动物中，抗坏血酸浓度在S3组分中最高（7.55±1.46微克抗坏血酸/毫克蛋白质），在P2p组分（神经末梢细胞质）中也相当高（5.37±0.91）。在抗坏血酸缺乏期间，P2p和微粒体组分中的含量下降速度略快于其他组分。即使在缺乏18天后，大脑中的乙酰胆碱含量也未发现显著变化。