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卷棘口吸虫(吸虫纲)成虫中磷脂的薄层色谱分析

Thin-layer chromatographic analysis of phospholipids in Echinostoma revolutum (Trematoda) adults.

作者信息

Fried B, Shapiro I L

出版信息

J Parasitol. 1979 Apr;65(2):243-5.

PMID:448610
Abstract

Thin-layer chromatographic analyses were made of phospholipids extracted from adult Echinostoma revolutum (Trematoda) and from the nonnutrient medium in which worms were maintained. Identity of phospholipids was based on comparison with authentic lipid standards and on specific chemical detection tests. The most abundant phospholipids in the extract were phosphatidyl choline, phosphatidyl ethanolamine and phosphatidyl serine; lesser amounts of cerebrosides and lysophosphatidyl choline were detected also. Phospholipids detected in the incubation medium were essentially as described for extracts.

摘要

对从成年卷棘口吸虫(吸虫纲)中提取的磷脂以及培养这些虫体的无营养培养基进行了薄层色谱分析。磷脂的鉴定基于与标准脂质真品的比较以及特定的化学检测试验。提取物中最丰富的磷脂是磷脂酰胆碱、磷脂酰乙醇胺和磷脂酰丝氨酸;还检测到了少量的脑苷脂和溶血磷脂酰胆碱。在培养液中检测到的磷脂基本上与提取物中描述的相同。

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