Bayer B M, Kruth H S, Vaughan M, Beaven M A
J Pharmacol Exp Ther. 1979 Jul;210(1):106-11.
Indomethacin reversibly inhibited growth of rat hepatoma cells (HTC) and human diploid fibroblasts in the G1 phase of the cell cycle. Cytophotometric measurements showed that greater than 90% of cells incubated for 48 hr with indomethacin had a DNA content that corresponded to the G1 state. Synchronous growth of both the HTC and fibroblast cultures occurred after removal of drug as indicated by the sequence of changes in [3H]thymidine incorporation into DNA, cellular DNA content, mitotic index and cell number. Autoradiographs of HTC cell cultures incubated with [3H]thymidine indicated that all (98%) of the cells engaged in DNA synthesis following the removal of indomethacin. Since viability of the cells was not impaired, even by prolonged exposure to indomethacin, this drug provides a means of synchronizing growth. Suppression of cell proliferation could contribute to the therapeutic and/or toxic effects of indomethacin in vivo.
吲哚美辛可逆性抑制大鼠肝癌细胞（HTC）和人二倍体成纤维细胞在细胞周期G1期的生长。细胞光度测量显示，用吲哚美辛孵育48小时的细胞中，超过90%的细胞DNA含量与G1期状态相对应。如[3H]胸苷掺入DNA、细胞DNA含量、有丝分裂指数和细胞数量的变化顺序所示，去除药物后HTC和成纤维细胞培养物均出现同步生长。用[3H]胸苷孵育的HTC细胞培养物的放射自显影片表明，去除吲哚美辛后，所有（98%）细胞都参与了DNA合成。由于即使长时间暴露于吲哚美辛，细胞活力也未受损，因此该药物提供了一种同步生长的方法。细胞增殖的抑制可能有助于吲哚美辛在体内的治疗和/或毒性作用。
