Whorton E B, Bee D E, Benge M C, Kilian D J
Mutat Res. 1979 Apr;64(2):87-9. doi: 10.1016/0165-1161(79)90003-7.
In a large multilaboratory cytogenetic study of interlaboratory variations using 4 dose levels of triethylenemelamine and a control, a severely damaged cell was operationally defined as a cell which contained at least 10 aberrations of any type. A review of this study suggested that the use of this definition introduced a bias in the measurement and interpretation of results for the other cytogenetic categories studied. As a result, the original severely damaged cells were carefully reanalyzed to investigate the characteristics of this bias and to seek procedures to minimize or eliminate it. Results characterize this bias and demonstrate that when a severely damaged cell is defined as one containing at least 20 aberrations and those aberrations in the remaining non-severely damaged cells are classified by specific type, the bias is significantly reduced and chromosome analysis can be improved as a test system.
在一项使用4种剂量水平的三乙烯三聚氰胺及一个对照组进行实验室间差异研究的大型多实验室细胞遗传学研究中，一个严重受损细胞在操作上被定义为含有至少10种任何类型畸变的细胞。对该研究的回顾表明，使用这一定义在对所研究的其他细胞遗传学类别结果的测量和解释中引入了偏差。因此，对最初的严重受损细胞进行了仔细的重新分析，以研究这种偏差的特征，并寻求将其最小化或消除的程序。结果描述了这种偏差的特征，并表明当一个严重受损细胞被定义为含有至少20种畸变的细胞，且其余未严重受损细胞中的畸变按特定类型分类时，偏差会显著降低，并且作为一种检测系统的染色体分析可以得到改进。
