Kilian D J, Moreland F M, Benge M C, Legator M S, Whorton E B
Mutat Res. 1977 Jul;44(1):97-104. doi: 10.1016/0027-5107(77)90118-x.
Six cytogenetics laboratories joined in a collaborative study of rat chromosome aberrations to measure interlaboratory variation in results of standardized procedures and to devise methods to minimize interlaboratory differences. A preliminary workshop was held to resolve scoring differences, to develop a joint protocol and common glossary, and to reach agreement on uniform reporting methods. Osborne-Mendel rats from a common source were sent to each laboratory. Triethylenemelamine (TEM) was used at doses of 100, 200, 300 and 400 microgram/kg to induce clastogenic effects; results were compared to those of a control group of untreated animals. Femoral bone marrow cells were evaluated with the scorers unaware of the dosage. Final results showed highly significant dose effects with the test compound, and most laboratories showed a similar pattern of dose response. This study illustrates that rat cytogenetic analysis can be an effective test system for evaluation of a compound for mutagenic potential, particularly for the index reflecting the proportion of abnormal cells, but that results should be interpreted cautiously when arbitrary values are assigned for some of the categories being analyzed, as was done in this project for the category of severely damaged cells.
六个细胞遗传学实验室参与了一项关于大鼠染色体畸变的合作研究，以测量标准化程序结果中的实验室间差异，并设计方法将实验室间差异降至最低。举办了一次初步研讨会，以解决评分差异、制定联合方案和通用术语表，并就统一报告方法达成一致。来自同一来源的奥斯本-孟德尔大鼠被送往每个实验室。使用剂量为100、200、300和400微克/千克的三乙烯三聚氰胺（TEM）来诱导致断裂效应；将结果与未处理动物的对照组进行比较。由不知道剂量的评分员对股骨骨髓细胞进行评估。最终结果显示，受试化合物具有高度显著的剂量效应，大多数实验室显示出相似的剂量反应模式。这项研究表明，大鼠细胞遗传学分析可以作为评估化合物致突变潜力的有效测试系统，特别是对于反映异常细胞比例的指标，但是当为某些被分析类别指定任意值时，结果应谨慎解释，本项目中对严重受损细胞类别就是如此。
