核糖核酸酶-A作用第一步的几何结构（内式几何结构——尿苷2',3'-环硫代磷酸酯——31P核磁共振）
Geometry of the first step in the action of ribonuclease-A (in-line geometry-uridine2',3'-cyclic thiophosphate- 31 P NMR).
作者信息
Usher D A, Erenrich E S, Eckstein F
出版信息
Proc Natl Acad Sci U S A. 1972 Jan;69(1):115-8. doi: 10.1073/pnas.69.1.115.
Abstract
Advantage was taken of the reversibility of the first step of ribonuclease-A action to synthesize the dinucleoside phosphorothioate Up(S)C from the crystalline isomer of uridine 2',3'-cyclic phosphorothioate [Up(S)] and cytidine. Cyclic phosphorothioate was then reformed from Up(S)C by a nonenzymic reaction known to proceed by an in-line mechanism. The geometry of the enzymic reaction was determined to be in-line by a comparison of the Up(S) product with the Up(S) originally used. By the principle of microscopic reversibility, the geometry of the first step in the action of ribonuclease-A is shown to be in-line.
摘要
利用核糖核酸酶A作用第一步的可逆性，从尿苷2',3'-环硫代磷酸酯[Up(S)]的结晶异构体和胞苷合成了二核苷硫代磷酸酯Up(S)C。然后通过已知按内式机制进行的非酶反应，由Up(S)C重新形成环硫代磷酸酯。通过将Up(S)产物与最初使用的Up(S)进行比较，确定酶促反应的几何形状为内式。根据微观可逆性原理，核糖核酸酶A作用第一步的几何形状显示为内式。
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