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2
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本文引用的文献

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Artificial enzymes.人工酶
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Neutron diffraction of crystalline proteins.晶体蛋白质的中子衍射。
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The role of mobility in the substrate binding and catalytic machinery of enzymes.流动性在酶的底物结合和催化机制中的作用。
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The refined crystal structure of ribonuclease A at 2.0 A resolution.核糖核酸酶A在2.0埃分辨率下的精细晶体结构。
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Orientation of histidine residues in RNase A: neutron diffraction study.核糖核酸酶A中组氨酸残基的取向：中子衍射研究
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[13C]Methylated ribonuclease A. 13C NMR studies of the interaction of lysine 41 with active site ligands.[13C]甲基化核糖核酸酶A。赖氨酸41与活性位点配体相互作用的13C核磁共振研究。
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Absolute stereochemistry of the second step of ribonuclease action.
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The three-dimensional structure of ribonuclease-S. Interpretation of an electron density map at a nominal resolution of 2 A.核糖核酸酶-S的三维结构。标称分辨率为2埃的电子密度图解读。
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Possible transition-state analogs for ribonuclease. The complexes of uridine with oxovanadium(IV) ion and vanadium(V) ion.核糖核酸酶可能的过渡态类似物。尿苷与氧钒（IV）离子和钒（V）离子的配合物。
J Am Chem Soc. 1973 Dec 26;95(26):8762-8. doi: 10.1021/ja00807a043.
10
Geometry of the first step in the action of ribonuclease-A (in-line geometry-uridine2',3'-cyclic thiophosphate- 31 P NMR).核糖核酸酶-A作用第一步的几何结构（内式几何结构——尿苷2',3'-环硫代磷酸酯——31P核磁共振）
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核糖核酸酶的活性位点：与钒酸尿苷（一种过渡态类似物）复合物的中子衍射研究

Active site of RNase: neutron diffraction study of a complex with uridine vanadate, a transition-state analog.

作者信息

Wlodawer A, Miller M, Sjölin L

出版信息

Proc Natl Acad Sci U S A. 1983 Jun;80(12):3628-31. doi: 10.1073/pnas.80.12.3628.

DOI:10.1073/pnas.80.12.3628
PMID:6574501
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC394103/
Abstract

A complex of RNase A with a transition-state analog, uridine vanadate, has been studied by a combination of neutron and x-ray diffraction. The vanadium atom occupies the center of a distorted trigonal bipyramid, with the ribose oxygen O2' at the apical position. Contrary to expectations based on the straightforward interpretation of the known in-line mechanism of action of RNase, nitrogen NE2 of histidine-12 was found to form a hydrogen bond to the equatorial oxygen O8, while nitrogen NZ of lysine-41 makes a clear hydrogen bond to the apical oxygen O2'. Nitrogen ND1 of histidine-119 appears to be within a hydrogen-bond distance of the other apical oxygen, O7. Two other hydrogen bonds between the vanadate and the protein are made by nitrogen NE2 of glutamine-11 and by the amide nitrogen of phenylalanine-120. The observed geometry of the complex may necessitate reinterpretation of the mechanism of action of RNase.

摘要

通过中子衍射和X射线衍射相结合的方法，对核糖核酸酶A与过渡态类似物钒酸尿苷形成的复合物进行了研究。钒原子位于一个扭曲的三角双锥的中心，核糖氧原子O2'处于顶端位置。与基于核糖核酸酶已知的直接作用机制的简单解释所预期的情况相反，发现组氨酸-12的氮原子NE2与赤道氧原子O8形成氢键，而赖氨酸-41的氮原子NZ与顶端氧原子O2'形成明显的氢键。组氨酸-119的氮原子ND1似乎处于与另一个顶端氧原子O7的氢键距离内。钒酸盐与蛋白质之间的另外两个氢键分别由谷氨酰胺-11的氮原子NE2和苯丙氨酸-120的酰胺氮原子形成。观察到的复合物几何结构可能需要对核糖核酸酶的作用机制进行重新解释。