Amos L A, Baker T S
Nature. 1979 Jun 14;279(5714):607-12. doi: 10.1038/279607a0.
A three-dimensional image of tubulin protofilaments, reconstructed to 20 A resolution from electron micrographs of negatively stained zinc-induced sheets, has been used to generate an improved model for microtubule substructure. This model has been used to phase a reconstructed image from X-ray amplitudes, which is compatible with the electron microscope results.
通过对负染锌诱导片层的电子显微照片进行重建,得到了分辨率为20埃的微管蛋白原丝三维图像,该图像被用于生成改进的微管亚结构模型。此模型已用于根据X射线振幅对重建图像进行相位分析,所得结果与电子显微镜结果相符。