DNA哺乳动物病毒的病毒致癌机制：腺病毒转化细胞中含有病毒和高度重复细胞碱基序列的RNA转录本（DNA-RNA杂交-病毒-细胞mRNA）
The mechanism of viral carcinogenesis by DNA mammalian viruses: RNA transcripts containing viral and highly reiterated cellular base sequences in adenovirus-transformed cells (DNA-RNA hybridization-viral-cell mRNA).
作者信息
Tsuei D, Fujinaga K, Green M
出版信息
Proc Natl Acad Sci U S A. 1972 Feb;69(2):427-30. doi: 10.1073/pnas.69.2.427.
Abstract
Virus-specific RNA was isolated from cells transformed by human adenovirus 2 and 7 by multiple hybridizations with and elutions from homologous viral DNA; RNA molecules purified by this selection procedure hybridized efficiently with both viral DNA (24-50%) and DNA from untransformed cells (12-27%). Virus-specific RNA isolated in the same manner from cells productively infected with adenoviruses did not hybridize significantly with cellular DNA. These findings suggest that RNA molecules containing covalently-linked viral and cellular sequences are transcribed in cells transformed by human adenoviruses. The high efficiency of hybridization with DNA from untransformed cells implies that viral DNA is integrated adjacent to highly reiterated cellular DNA sequences.
摘要
通过与同源病毒DNA多次杂交并从其洗脱，从人腺病毒2型和7型转化的细胞中分离出病毒特异性RNA；通过这种筛选程序纯化的RNA分子能与病毒DNA（24%-50%）和未转化细胞的DNA（12%-27%）高效杂交。以同样方式从被腺病毒有效感染的细胞中分离出的病毒特异性RNA与细胞DNA无明显杂交。这些发现表明，在人腺病毒转化的细胞中，转录出了含有共价连接的病毒和细胞序列的RNA分子。与未转化细胞的DNA高效杂交意味着病毒DNA整合在高度重复的细胞DNA序列附近。
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引用本文的文献
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2
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Mechanism of viral carcinogenesis by DNA mammalian viruses. V. Properties of purified viral-specific RNA from human adenovirus-induced tumor cells.DNA 哺乳动物病毒引发病毒致癌的机制。V. 来自人腺病毒诱导肿瘤细胞的纯化病毒特异性RNA的特性
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Mechanism of viral carcinogenesis by DNA mammalian viruses. VII. Viral genes transcribed in adenovirus type 2 infected and transformed cells.DNA 哺乳动物病毒的病毒致癌机制。VII. 在 2 型腺病毒感染和转化细胞中转录的病毒基因。
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