Tsuei D, Fujinaga K, Green M
Proc Natl Acad Sci U S A. 1972 Feb;69(2):427-30. doi: 10.1073/pnas.69.2.427.
Virus-specific RNA was isolated from cells transformed by human adenovirus 2 and 7 by multiple hybridizations with and elutions from homologous viral DNA; RNA molecules purified by this selection procedure hybridized efficiently with both viral DNA (24-50%) and DNA from untransformed cells (12-27%). Virus-specific RNA isolated in the same manner from cells productively infected with adenoviruses did not hybridize significantly with cellular DNA. These findings suggest that RNA molecules containing covalently-linked viral and cellular sequences are transcribed in cells transformed by human adenoviruses. The high efficiency of hybridization with DNA from untransformed cells implies that viral DNA is integrated adjacent to highly reiterated cellular DNA sequences.
通过与同源病毒DNA多次杂交并从其洗脱，从人腺病毒2型和7型转化的细胞中分离出病毒特异性RNA；通过这种筛选程序纯化的RNA分子能与病毒DNA（24%-50%）和未转化细胞的DNA（12%-27%）高效杂交。以同样方式从被腺病毒有效感染的细胞中分离出的病毒特异性RNA与细胞DNA无明显杂交。这些发现表明，在人腺病毒转化的细胞中，转录出了含有共价连接的病毒和细胞序列的RNA分子。与未转化细胞的DNA高效杂交意味着病毒DNA整合在高度重复的细胞DNA序列附近。
