由2型腺病毒转化的大鼠胚胎细胞核与细胞质中的病毒特异性核糖核酸
Virus-specific ribonucleic acid in the nucleus and cytoplasm of rat embryo cells transformed by adenovirus type 2.
作者信息
Shimada K, Fujinaga K, Hama S, Sekikawa K, Ito Y
出版信息
J Virol. 1972 Oct;10(4):648-52. doi: 10.1128/JVI.10.4.648-652.1972.
Abstract
Nuclei were isolated from rat embryo cells transformed by adenovirus type 2. Nuclear and cytoplasmic virus-specific ribonucleic acids (RNA) were characterized and quantitated by deoxyribonucleic acid (DNA)-RNA hybrid formation with adenovirus DNA. The results indicate that most, if not all, virus-specific RNA molecules are synthesized in the cell nucleus and subsequently transported into cytoplasm where they degrade with a half-life of 1 to 2 hr. No difference in base sequences between nuclear and cytoplasmic virus-specific RNA species can be detected by hybridization competition experiment with viral DNA.
摘要
从经2型腺病毒转化的大鼠胚胎细胞中分离出细胞核。通过用腺病毒DNA进行脱氧核糖核酸（DNA）-核糖核酸（RNA）杂交形成，对细胞核和细胞质中的病毒特异性核糖核酸（RNA）进行了表征和定量。结果表明，大多数（如果不是全部）病毒特异性RNA分子是在细胞核中合成的，随后转运到细胞质中，在那里它们以1至2小时的半衰期降解。通过与病毒DNA的杂交竞争实验，未检测到细胞核和细胞质中病毒特异性RNA种类之间的碱基序列差异。
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