Jennings R, Freeman M J
J Hyg (Lond). 1972 Mar;70(1):1-11. doi: 10.1017/s0022172400022038.
The effect of varying conditions of inoculation and incubation on the growth of type C influenza virus in the allantoic cavity of the developing chick embryo were investigated. It was found that the highest yields of both virus haemagglutinin and infectious virus were obtained following the inoculation of chick embryos at 8 days with subsequent incubation at 32 degrees C. Using the chick embryo allantoic cavity for titration of infectious virus, growth curves of allantoically propagated virus under varying inoculation and incubation conditions were determined.
研究了接种和培养条件的变化对C型流感病毒在发育中的鸡胚尿囊腔中生长的影响。结果发现,在8日龄鸡胚接种后于32℃培养,可获得最高的病毒血凝素产量和感染性病毒产量。利用鸡胚尿囊腔对感染性病毒进行滴定,测定了在不同接种和培养条件下尿囊传播病毒的生长曲线。