培养的人淋巴母细胞中磷酸核糖基转移酶基因座的化学诱变
Chemical mutagenesis at the phosphoribosyltransferase locus in cultured human lymphoblasts.
作者信息
Sato K, Slesinski R S, Littlefield J W
出版信息
Proc Natl Acad Sci U S A. 1972 May;69(5):1244-8. doi: 10.1073/pnas.69.5.1244.
Abstract
The presence of selectable genetic markers in long-term human lymphoblast cultures would facilitate cell hybridization experiments on the biosynthesis of immunoglobulins, as well as other studies. This work reports the induction with ethylmethane sulfonate of 6-thioguanine - resistant, phosphoribosyltransferase - deficient mutants in a lymphoblast line from a patient with infectious mononucleosis. These cells were unusually sensitive, with a D(0) value of 28 mug of ethylmethane sulfonate per ml; the sensitivity curve followed a biphasic pattern suggesting the presence of 3% resistant cells. Ethylmethane sulfonate increased the frequency of mutants resistant to 6-thioguanine over 100-fold, to about 2 x 10(-4); nitrosoguanidine was less effective. Almost all the mutants contained considerably less than 1% of the hypoxanthine-guanine phosphoribosyltransferase (EC 2.4.2.8) activity of wild-type cells. The mutation did not appear to result from loss of an X chromosome.
摘要
在长期培养的人淋巴母细胞中存在可选择的遗传标记，将有助于进行关于免疫球蛋白生物合成的细胞杂交实验以及其他研究。本文报道了用甲磺酸乙酯诱导来自一名传染性单核细胞增多症患者的淋巴母细胞系中对6-硫鸟嘌呤耐药、磷酸核糖基转移酶缺陷的突变体。这些细胞异常敏感，甲磺酸乙酯的D(0)值为每毫升28微克；敏感性曲线呈双相模式，表明存在3%的耐药细胞。甲磺酸乙酯使对6-硫鸟嘌呤耐药的突变体频率增加了100多倍，达到约2×10⁻⁴；亚硝基胍的效果较差。几乎所有突变体所含的次黄嘌呤-鸟嘌呤磷酸核糖基转移酶（EC 2.4.2.8）活性都大大低于野生型细胞的1%。该突变似乎不是由X染色体缺失导致的。
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本文引用的文献
1
Diploid azaguanine-resistant mutants of cultured human fibroblasts.培养的人成纤维细胞的二倍体氮杂鸟嘌呤抗性突变体。
Science. 1970 Jul 31;169(3944):482-5. doi: 10.1126/science.169.3944.482.
2
Protein measurement with the Folin phenol reagent.使用福林酚试剂进行蛋白质测定。
J Biol Chem. 1951 Nov;193(1):265-75.
3
CONTINUOUS CULTURE OF HUMAN LYMPHOBLASTS FROM PERIPHERAL BLOOD OF A CHILD WITH ACUTE LEUKEMIA.从一名急性白血病患儿外周血中连续培养人淋巴细胞
Cancer. 1965 Apr;18:522-9. doi: 10.1002/1097-0142(196504)18:4<522::aid-cncr2820180418>3.0.co;2-j.
4
THE INOSINIC ACID PYROPHOSPHORYLASE ACTIVITY OF MOUSE FIBROBLASTS PARTIALLY RESISTANT TO 8-AZAGUANINE.对8-氮杂鸟嘌呤有部分抗性的小鼠成纤维细胞的肌苷酸焦磷酸化酶活性
Proc Natl Acad Sci U S A. 1963 Sep;50(3):568-73. doi: 10.1073/pnas.50.3.568.
5
Deoxyriboside control and synchronization of mitosis.脱氧核糖核苷对有丝分裂的控制与同步化
Nature. 1962 May 19;194:682-3. doi: 10.1038/194682a0.
6
The serial cultivation of human diploid cell strains.人二倍体细胞株的连续培养。
Exp Cell Res. 1961 Dec;25:585-621. doi: 10.1016/0014-4827(61)90192-6.
7
The relation of radioresistance to budding in Saccharomyces cerevisiae.酿酒酵母中抗辐射性与出芽的关系。
Arch Biochem Biophys. 1954 Mar;49(1):110-22. doi: 10.1016/0003-9861(54)90172-1.
8
The cloning of normal "mast" cells in tissue culture.组织培养中正常“肥大”细胞的克隆。
J Cell Physiol. 1965 Dec;66(3):319-24. doi: 10.1002/jcp.1030660309.
9
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Am J Dis Child. 1969 Feb;117(2):129-36. doi: 10.1001/archpedi.1969.02100030131001.
10
Infectious mononucleosis: continuous suspension culture of peripheral blood leucocytes.
Nature. 1968 Feb 10;217(5128):564-5. doi: 10.1038/217564a0.
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