哺乳动物细胞中的8-氮杂鸟嘌呤抗性。I. 次黄嘌呤-鸟嘌呤磷酸核糖转移酶
8-Azaguanine resistance in mammalian cells. I. Hypoxanthine-guanine phosphoribosyltransferase.
作者信息
Gillin F D, Roufa D J, Beaudet A L, Caskey C T
出版信息
Genetics. 1972 Oct;72(2):239-52. doi: 10.1093/genetics/72.2.239.
Abstract
Chinese hamster cells were treated with ethyl methanesulfonate or N-methyl-N'-nitro-N-nitrosoguanidine, and mutants resistant to 8-azaguanine were selected and characterized. Hypoxanthine-guanine phosphoribosyltransferase activity of sixteen mutants is extremely negative, making them suitable for reversion to HGPRTase(+). Ten of the extremely negative mutants revert at a frequency higher than 10(-7) suggesting their point mutational character. The remaining mutants have demonstrable HGPRTase activity and are not useful for reversion analysis. Five of these mutants have < 2% HGPRTase and are presumably also HGPRTase point mutants. The remaining 14 mutants utilize exogenous hypoxanthine for nucleic acid synthesis poorly, and possess 20-150% of wild-type HGPRTase activity in in vitro. Their mechanism of 8-azaguanine resistance is not yet defined.
摘要
用甲磺酸乙酯或N-甲基-N'-硝基-N-亚硝基胍处理中国仓鼠细胞，然后筛选并鉴定对8-氮杂鸟嘌呤具有抗性的突变体。16个突变体的次黄嘌呤-鸟嘌呤磷酸核糖转移酶活性极低，这使得它们适合回复为HGPRTase(+)。其中10个极低活性突变体的回复频率高于10(-7)，表明它们具有点突变特征。其余的突变体具有可检测到的HGPRTase活性，不适合用于回复分析。这些突变体中有5个的HGPRTase活性小于2%，推测也是HGPRTase点突变体。其余14个突变体利用外源性次黄嘌呤进行核酸合成的能力较差，在体外具有野生型HGPRTase活性的20%-150%。它们对8-氮杂鸟嘌呤的抗性机制尚未明确。
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本文引用的文献
1
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Biochem J. 1965 Jan;94(1):71-4. doi: 10.1042/bj0940071.
2
BIOCHEMICALLY MARKED VARIANTS OF THE SYRIAN HAMSTER FIBROBLAST CELL LINE BHK21 AND ITS DERIVATIVES.叙利亚仓鼠成纤维细胞系BHK21及其衍生物的生化标记变体。
Exp Cell Res. 1965 Apr;38:106-19. doi: 10.1016/0014-4827(65)90432-5.
3
4
5
6
Decreased feedback inhibition in a 6-(methylmercapto)purine ribonucleoside-resistant tumor.6-（甲硫基）嘌呤核糖核苷抗性肿瘤中反馈抑制的降低
Cancer Res. 1967 Oct;27(10):1773-8.
7
Inheritance of purine phosphoribosyltransferases in man.人类嘌呤磷酸核糖转移酶的遗传
Am J Hum Genet. 1969 Jan;21(1):61-70.
8
9
The relationship of gene structure and protein structure of iso-I-cytochrome c from yeast.酵母同工酶I-细胞色素c的基因结构与蛋白质结构的关系。
Symp Soc Exp Biol. 1970;24:85-107.
10
Classification of aminotransferase (C gene) mutants in the histidine operon.组氨酸操纵子中氨基转移酶（C基因）突变体的分类
J Mol Biol. 1966 Nov 14;21(2):335-55. doi: 10.1016/0022-2836(66)90103-3.
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