大分子中荧光偏振的动力学
Dynamics of fluorescence polarization in macromolecules.
作者信息
Belford G G, Belford R L, Weber G
出版信息
Proc Natl Acad Sci U S A. 1972 Jun;69(6):1392-3. doi: 10.1073/pnas.69.6.1392.
Abstract
Reexamination of the theory of fluorescence time dependence owing to rotational diffusion of rigid macromolecules reveals deficiencies or hidden restrictions in each of the previous treatments. The correct master equation has five exponential decay terms, with preexponential factors that depend upon the diffusion constants and, in a completely symmetrical fashion, upon the orientations of absorbing and emitting dipoles.
摘要
对由于刚性大分子的旋转扩散导致的荧光时间依赖性理论的重新审视揭示了之前每种处理方法中的缺陷或隐藏限制。正确的主方程有五个指数衰减项，其指数前因子取决于扩散常数，并且以完全对称的方式取决于吸收和发射偶极子的取向。
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