Hartmann J F, Gwatkin R B, Hutchison C F
Proc Natl Acad Sci U S A. 1972 Oct;69(10):2767-9. doi: 10.1073/pnas.69.10.2767.
A study in vitro of interactions between gametes of the golden hamster showed that the spermatozoon associates with the zona pellucida, or outer coat of the ovum, by two successive steps termed attachment and binding. Attachment, apparently the first step in fertilization, is not species-specific; it is insensitive to temperature (2 degrees ) and is reversible. Binding, on the other hand, is species-specific, temperature-sensitive, and irreversible. Experiments with isolated zonae pellucidae indicated that the vitellus (cellular portion of the ovum) influences the interaction between the sperm and the zona pellucida by increasing the time required for the sperm to bind. This effect is exerted on the sperm while they are attached to the zona pellucida.
一项关于金黄地鼠配子间相互作用的体外研究表明，精子通过两个连续步骤与透明带（即卵子的外层包膜）结合，这两个步骤分别称为附着和黏附。附着显然是受精的第一步，它不具有物种特异性；对温度（2摄氏度）不敏感且是可逆的。另一方面，黏附则具有物种特异性、对温度敏感且是不可逆的。对分离出的透明带进行的实验表明，卵黄（卵子的细胞部分）通过增加精子黏附所需的时间来影响精子与透明带之间的相互作用。这种作用是在精子附着于透明带时施加于精子的。
