聚戊烯醇结合糖在肝脏糖蛋白合成中作为中间体的作用。
The role of polyprenol-bound saccharides as intermediates in glycoprotein synthesis in liver.
作者信息
Parodi A J, Behrens N H, Leloir L F, Carminatti H
出版信息
Proc Natl Acad Sci U S A. 1972 Nov;69(11):3268-72. doi: 10.1073/pnas.69.11.3268.
It has been reported that liver microsomes catalyze the transfer of glucose from uridine diphosphate glucose to dolichol monophosphate so as to produce dolichol monophosphate glucose. Dolichol is a polyprenol containing about 20 isoprene units. The glucosyl residue of dolichol monophosphate glucose is transferred to an endogenous acceptor on further incubation with liver microsomes. The glucosylated endogenous acceptor appears to be an oligosaccharide of about 20 monosaccharide units bound to dolichol through a phosphate or pyrophosphate bridge. In this paper it is reported that liver microsomes catalyze the transfer of the oligosaccharide from the glucosylated endogenous acceptor to an endogenous protein. This transfer reaction requires the presence of bivalent cations, manganese being more effective than magnesium. The presence of deoxycholate is also required. Besides the glycoprotein, several water-soluble products are also formed. Preliminary evidence indicates that they are glucose, iligosaccharides of different size, and possibly oligosaccharides bound to amino acids.
据报道，肝微粒体催化葡萄糖从尿苷二磷酸葡萄糖转移至磷酸多萜醇，从而生成磷酸多萜醇葡萄糖。多萜醇是一种含有约20个异戊二烯单元的聚异戊烯醇。磷酸多萜醇葡萄糖的葡萄糖基残基在与肝微粒体进一步温育时转移至内源性受体。糖基化的内源性受体似乎是一种通过磷酸或焦磷酸桥与多萜醇结合的约20个单糖单元的寡糖。本文报道肝微粒体催化寡糖从糖基化的内源性受体转移至内源性蛋白质。该转移反应需要二价阳离子的存在，锰比镁更有效。还需要脱氧胆酸盐的存在。除了糖蛋白外，还形成了几种水溶性产物。初步证据表明它们是葡萄糖、不同大小的寡糖，可能还有与氨基酸结合的寡糖。
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