含甘露糖的脂质结合寡糖的形成。它们在糖蛋白合成中的作用。
Formation of lipid-bound oligosaccharides containing mannose. Their role in glycoprotein synthesis.
作者信息
Behrens N H, Carminatti H, Staneloni R J, Leloir L F, Cantarella A I
出版信息
Proc Natl Acad Sci U S A. 1973 Dec;70(12):3390-4. doi: 10.1073/pnas.70.12.3390.
Abstract
Incubation of GDP-[(14)C]mannose with liver microsomes and magnesium ions led to the formation of dolichol monophosphate mannose and of other acidlabile compounds that contain oligosaccharides. Formation of these compounds was enhanced by addition of an acceptor lipid in the same fractions of DEAE-cellulose chromatography where bound dolichol is found. Alkaline treatment of the oligosaccharides, obtained by acid methanolysis, followed by paper electrophoresis, gave rise to the formation of two positively charged substances believed to be formed by deacetylation of hexosamine residues. Incubation of the oligosaccharide-containing compounds with liver microsomes and manganese ions resulted in a transfer to endogenous protein. The role of dolichol derivatives in glycoprotein synthesis is discussed.
摘要
将GDP-[(14)C]甘露糖与肝微粒体及镁离子一起孵育，导致形成了磷酸多萜醇甘露糖以及其他含有寡糖的酸不稳定化合物。在发现结合多萜醇的相同二乙氨基乙基纤维素色谱级分中加入受体脂质，可增强这些化合物的形成。对通过酸性甲醇解得到的寡糖进行碱性处理，然后进行纸电泳，产生了两种带正电荷的物质，据信它们是由己糖胺残基的脱乙酰化形成的。将含寡糖的化合物与肝微粒体及锰离子一起孵育，导致其转移至内源性蛋白质上。文中讨论了多萜醇衍生物在糖蛋白合成中的作用。
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