饥饿增强己糖进入鸡成纤维细胞的过程：对半乳糖和葡萄糖的不同影响。
Enhancement of hexose entry into chick fibroblasts by starvation: differential effect on galactose and glucose.
作者信息
Martineau R, Kohlbacher M, Shaw S N, Amos H
出版信息
Proc Natl Acad Sci U S A. 1972 Nov;69(11):3407-11. doi: 10.1073/pnas.69.11.3407.
Abstract
Glucose entry, as measured by 5-min uptake into the acid-soluble fraction, is enhanced 15-30 times by long-term (12-24 hr) hexose starvation of chick fibroblasts. The rate of galactose accumulation in the cells increases only 5 times under the same conditions of starvation. Several carbon and energy sources that were tested for their effect on this "derepression" can be classified as: (i) those resembling glucose in blocking the "stimulation," (ii) those permitting full "derepression"; and (iii) those partially preventing the enhanced entry. Inhibitors of protein synthesis block enhancement under conditions otherwise conducive to it. We conclude that the glucose and galactose carrier systems are not identical, based largely on the asymmetric "repression" observed when glucose and galactose are compared as "repressors."
摘要
通过测定5分钟内进入酸溶性部分的摄取量来衡量，长期（12 - 24小时）对鸡成纤维细胞进行己糖饥饿处理后，葡萄糖的摄取增强了15 - 30倍。在相同的饥饿条件下，细胞中半乳糖的积累速率仅增加了5倍。测试了几种碳源和能源对这种“去阻遏”的影响，可将它们分为：（i）在阻断“刺激”方面类似于葡萄糖的物质；（ii）允许完全“去阻遏”的物质；以及（iii）部分阻止增强摄取的物质。蛋白质合成抑制剂在其他有利于增强摄取的条件下会阻断这种增强作用。我们得出结论，葡萄糖和半乳糖载体系统并不相同，这主要基于将葡萄糖和半乳糖作为“阻遏物”进行比较时观察到的不对称“阻遏”现象。
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