用来自不同物种脑或肝的纯化及部分纯化组分对兔血红蛋白信使核糖核酸进行体外翻译。

Translation of rabbit hemoglobin meessenger RNA in vitro with purified and partially purified components from brain or liver of different species.

作者信息

Schreier M H, Staehelin T

出版信息

Proc Natl Acad Sci U S A. 1973 Feb;70(2):462-5. doi: 10.1073/pnas.70.2.462.

DOI:10.1073/pnas.70.2.462

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC433283/

Abstract

Two mammalian systems have been developed for efficient in vitro translation of exogenous messenger RNA (rabbit globin mRNA). One system is completely derived from guinea pig brain, and the other is from liver of different species. Both systems consist of purified 60S and 40S ribosomal sununits, unseparated initiation factors partially purified by ammonium sulfate precipitation and DEAE-cellulose chromatography, and pH 5 enzyme fractions as sources of elongation and termination factors, aminoacyl-tRNA synthetases, and transfer RNA. Translation depends completely upon exogenous mRNA and initiation factors. Extraction of initiation factors from microsomes or ribosomes has been improved for these tissues by inclusion of Mg(++) ions in the 0.5 M KCl extraction solution. Both systems synthesize complete rabbit alpha- and beta-globin chains, but in variable ratios. The overall efficiencies of the two systems are about 60% (liver) and 40% (brain) of a comparable system with rabbit reticulocyte initiation factors.

摘要

已开发出两种哺乳动物系统用于外源信使核糖核酸（兔珠蛋白信使核糖核酸）的高效体外翻译。一种系统完全源自豚鼠脑，另一种来自不同物种的肝脏。两种系统均由纯化的60S和40S核糖体亚基、通过硫酸铵沉淀和DEAE - 纤维素色谱法部分纯化的未分离起始因子，以及作为延伸和终止因子、氨酰 - tRNA合成酶和转运RNA来源的pH 5酶组分组成。翻译完全依赖于外源信使核糖核酸和起始因子。通过在0.5M KCl提取溶液中加入Mg(++)离子，已改进了从这些组织的微粒体或核糖体中提取起始因子的方法。两种系统均能合成完整的兔α和β珠蛋白链，但比例不同。与具有兔网织红细胞起始因子的可比系统相比，这两种系统的总体效率分别约为60%（肝脏）和40%（脑）。

相似文献

1

Translation of rabbit hemoglobin meessenger RNA in vitro with purified and partially purified components from brain or liver of different species.用来自不同物种脑或肝的纯化及部分纯化组分对兔血红蛋白信使核糖核酸进行体外翻译。

Proc Natl Acad Sci U S A. 1973 Feb;70(2):462-5. doi: 10.1073/pnas.70.2.462.

2

Translation of exogenous messenger RNA for hemoglobin on reticulocyte and liver ribosomes.外源性信使核糖核酸在网织红细胞和肝脏核糖体上用于血红蛋白的翻译。

Proc Natl Acad Sci U S A. 1971 Nov;68(11):2752-6. doi: 10.1073/pnas.68.11.2752.

3

Discrimination between messenger ribonucleic acids by a mammalian translation initiation factor.哺乳动物翻译起始因子对信使核糖核酸的区分

Proc Natl Acad Sci U S A. 1973 Jul;70(7):2139-44. doi: 10.1073/pnas.70.7.2139.

4

Initiation of eukaryotic protein synthesis: (Met-tRNA f -40S ribosome) initiation complex catalysed by purified initiation factors in the absence of mRNA.真核生物蛋白质合成的起始：在没有mRNA的情况下，由纯化的起始因子催化的（甲硫氨酰 - tRNAf - 40S核糖体）起始复合物。

Nat New Biol. 1973 Mar 14;242(115):35-8. doi: 10.1038/newbio242035a0.

5

Translational control of hemoglobin synthesis by an initiation factor required for recycling of ribosomes and for their binding to messenger RNA.通过核糖体循环及其与信使核糖核酸结合所需的起始因子对血红蛋白合成进行的翻译控制。

Proc Natl Acad Sci U S A. 1972 Nov;69(11):3317-21. doi: 10.1073/pnas.69.11.3317.

6

Translation of duck-globin messenger RNA in a partially purified mammalian cell-free system.鸭珠蛋白信使核糖核酸在部分纯化的哺乳动物无细胞系统中的翻译。

Eur J Biochem. 1973 Apr;34(2):213-8. doi: 10.1111/j.1432-1033.1973.tb02748.x.

7

Two eukaryotic initiation factors (IF-I and IF-II) of protein synthesis that are required to form an initiation complex with rabbit reticulocyte ribosomes.两种蛋白质合成的真核起始因子（IF-I和IF-II），它们是与兔网织红细胞核糖体形成起始复合物所必需的。

Proc Natl Acad Sci U S A. 1974 Feb;71(2):436-40. doi: 10.1073/pnas.71.2.436.

8

The translation of rabbit hemoglobin messenger RNA in a cell-free extract from chick embryo brain.兔血红蛋白信使核糖核酸在鸡胚脑无细胞提取物中的翻译。

Dev Biol. 1974 Feb;36(2):299-310. doi: 10.1016/0012-1606(74)90053-0.

9

The role of initiation factors in hemoglobin synthesis.起始因子在血红蛋白合成中的作用。

Hamatol Bluttransfus. 1972;10:109-32.

10

Increased translation as a result of elevated initation factor activity after administration of 3-methylcholanthrene.给予3-甲基胆蒽后，由于起始因子活性升高导致翻译增加。

Adv Exp Med Biol. 1975;58(00):151-64. doi: 10.1007/978-1-4615-9026-2_10.

引用本文的文献

1

Yeast eIF4A enhances recruitment of mRNAs regardless of their structural complexity.酵母 eIF4A 增强了对 mRNAs 的募集，而不管其结构复杂性如何。

Elife. 2017 Nov 30;6:e31476. doi: 10.7554/eLife.31476.

2

Pre-translational control of the differentiated state: Test for free cytoplasmic Hb-Messenger ribonucleoprotein in chick erythroblasts and chick embryo brain.分化状态的转录前调控：鸡成红细胞和鸡胚脑中游离细胞质血红蛋白信使核糖核蛋白的检测

Wilehm Roux Arch Dev Biol. 1976 Mar;179(1):33-40. doi: 10.1007/BF00857638.

3

Quantitative studies of mRNA recruitment to the eukaryotic ribosome.真核生物核糖体mRNA募集的定量研究。

Biochimie. 2015 Jul;114:58-71. doi: 10.1016/j.biochi.2015.02.017. Epub 2015 Mar 2.

本文引用的文献

1

Ribosomal aggregate engaged in protein synthesis: characterization of the ergosome.参与蛋白质合成的核糖体聚集体：多核蛋白体的表征

Nature. 1963 Feb 2;197:430-5. doi: 10.1038/197430a0.

2

Assembly of the peptide chains of hemoglobin.血红蛋白肽链的组装。

Proc Natl Acad Sci U S A. 1961 Mar 15;47(3):247-61. doi: 10.1073/pnas.47.3.247.

3

Initiation of globin synthesis.珠蛋白合成的起始

FEBS Lett. 1972 Aug 15;24(3):310-314. doi: 10.1016/0014-5793(72)80379-x.

4

Structure and function of mammalian ribosomes. I. Isolation and characterization of active liver ribosomal subunits.哺乳动物核糖体的结构与功能。I. 活性肝脏核糖体亚基的分离与特性分析。

J Mol Biol. 1970 Oct 14;53(1):1-19. doi: 10.1016/0022-2836(70)90042-2.

5

Formation of the initiation complex using muscle messenger RNAs.利用肌肉信使核糖核酸形成起始复合物。

Nature. 1970 Feb 21;225(5234):696-8. doi: 10.1038/225696a0.

6

Translation of exogenous messenger RNA for hemoglobin on reticulocyte and liver ribosomes.外源性信使核糖核酸在网织红细胞和肝脏核糖体上用于血红蛋白的翻译。

Proc Natl Acad Sci U S A. 1971 Nov;68(11):2752-6. doi: 10.1073/pnas.68.11.2752.

7

Specificity of mRNA binding factor in eukaryotes.真核生物中mRNA结合因子的特异性。

Proc Natl Acad Sci U S A. 1970 Dec;67(4):1782-8. doi: 10.1073/pnas.67.4.1782.

8

Cell-free protein synthesis in mixed systems with components from ascites cells and reticulocytes.在含有腹水细胞和网织红细胞成分的混合系统中进行无细胞蛋白质合成。

Biochim Biophys Acta. 1971 Sep 30;247(1):133-40. doi: 10.1016/0005-2787(71)90816-1.

9

Hemoglobin messenger ribonucleic acid translation in cell-free systems from rat and mouse liver and Landschutz ascites cells.血红蛋白信使核糖核酸在大鼠、小鼠肝脏及兰施茨腹水癌细胞的无细胞体系中的翻译

Biochemistry. 1972 Sep 12;11(19):3636-40. doi: 10.1021/bi00769a022.

10

Translation of rabbit globin mRNA.兔珠蛋白信使核糖核酸的翻译

Nat New Biol. 1972 Aug 16;238(85):200-2. doi: 10.1038/newbio238200a0.

文献AI研究员

20分钟写一篇综述，助力文献阅读效率提升50倍。

用中文搜PubMed

大模型驱动的PubMed中文搜索引擎

文档翻译

学术文献翻译模型，支持多种主流文档格式。