通过核糖体循环及其与信使核糖核酸结合所需的起始因子对血红蛋白合成进行的翻译控制。
Translational control of hemoglobin synthesis by an initiation factor required for recycling of ribosomes and for their binding to messenger RNA.
作者信息
Kaempfer R, Kaufman J
出版信息
Proc Natl Acad Sci U S A. 1972 Nov;69(11):3317-21. doi: 10.1073/pnas.69.11.3317.
Abstract
The continued recycling of ribosomes during protein synthesis in rabbit reticulocyte lysates at 37 degrees requires an initiation factor whose activity is rapidly lost in the absence of added heme. Partially purified factor (i) fully maintains the polysomes; (ii) inhibits the association of 40S and 60S ribosomal subunits into single ribosomes; (iii) promotes the quantitative entry of added 60S subunits into polysomes; (iv) allows the accumulation of ribosomal subunits, instead of single ribosomes, when initiation is blocked with aurin tricarboxylate; and (v) is absolutely required for the binding of globin messenger RNA to ribosomes.These properties suggest that this mammalian initiation factor functions analogously to bacterial IF-3. In addition, the translational control of globin synthesis by heme is exerted, directly or indirectly, through this factor.
摘要
在37摄氏度条件下，兔网织红细胞裂解物中蛋白质合成过程中核糖体的持续循环需要一种起始因子，在没有添加血红素的情况下，该因子的活性会迅速丧失。部分纯化的因子（i）能完全维持多核糖体；（ii）抑制40S和60S核糖体亚基结合形成单个核糖体；（iii）促进添加的60S亚基定量进入多核糖体；（iv）当用金精三羧酸阻断起始时，允许核糖体亚基而非单个核糖体积累；（v）是珠蛋白信使RNA与核糖体结合所绝对必需的。这些特性表明，这种哺乳动物起始因子的功能类似于细菌的IF-3。此外，血红素对珠蛋白合成的翻译控制是直接或间接通过该因子实现的。
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Translation of exogenous messenger RNA for hemoglobin on reticulocyte and liver ribosomes.外源性信使核糖核酸在网织红细胞和肝脏核糖体上用于血红蛋白的翻译。
Proc Natl Acad Sci U S A. 1971 Nov;68(11):2752-6. doi: 10.1073/pnas.68.11.2752.
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