Propapain and its conversion to papain: a new type of zymogen activation mechanism involving intramolecular thiol-disulphide interchange.

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作者信息

Brocklehurst K, Kierstan M P

出版信息

Nat New Biol. 1973 Apr 11;242(119):167-70. doi: 10.1038/newbio242167a0.

DOI:10.1038/newbio242167a0

PMID:4512754

Abstract

摘要

相似文献

Propapain and its conversion to papain: a new type of zymogen activation mechanism involving intramolecular thiol-disulphide interchange.

Nat New Biol. 1973 Apr 11;242(119):167-70. doi: 10.1038/newbio242167a0.

Evidence for association-activation effects in reactions of papain from studies on its reactivity towards isomeric two-protonic-state reactivity probes.来自木瓜蛋白酶对异构双质子态反应性探针反应性研究的关联激活效应证据。

Biochem J. 1979 Nov 1;183(2):369-73. doi: 10.1042/bj1830369.

Evidence that binding to the s2-subsite of papain may be coupled with catalytically relevant structural change involving the cysteine-25-histidine-159 diad. Kinetics of the reaction of papain with a two-protonic-state reactivity probe containing a hydrophobic side chain.有证据表明，与木瓜蛋白酶的s2亚位点结合可能与涉及半胱氨酸-25-组氨酸-159二元组的催化相关结构变化相关联。木瓜蛋白酶与含有疏水侧链的双质子态反应性探针反应的动力学。

Biochem J. 1979 Nov 1;183(2):223-31. doi: 10.1042/bj1830223.

Production and recovery of recombinant propapain with high yield.高产重组木瓜蛋白酶的生产与回收

Phytochemistry. 2009 Mar;70(4):465-72. doi: 10.1016/j.phytochem.2009.02.001. Epub 2009 Mar 9.

Active papain renatured and processed from insoluble recombinant propapain expressed in Escherichia coli.

Protein Eng. 1992 Jul;5(5):455-9. doi: 10.1093/protein/5.5.455.

Identification of signalling and non-signalling binding contributions to enzyme reactivity. Alternative combinations of binding interactions provide for change in transition-state geometry in reactions of papain.确定信号和非信号结合对酶反应性的贡献。结合相互作用的不同组合导致木瓜蛋白酶反应中过渡态几何结构的变化。

Biochem J. 1989 Mar 15;258(3):755-64. doi: 10.1042/bj2580755.

Characterization of the papain active centre by using two-protonic-state electrophiles as reactivity probes. Evidence for nucleophilic reactivity in the un-interrupted cysteine-25-histidine-159 interactive system.使用双质子态亲电试剂作为反应性探针表征木瓜蛋白酶活性中心。在不间断的半胱氨酸-25-组氨酸-159相互作用系统中亲核反应性的证据。

Biochem J. 1978 May 1;171(2):385-401. doi: 10.1042/bj1710385.

Structure of chymopapain M the late-eluted chymopapain deduced by comparative modelling techniques and active-centre characteristics determined by pH-dependent kinetics of catalysis and reactions with time-dependent inhibitors: the Cys-25/His-159 ion-pair is insufficient for catalytic competence in both chymopapain M and papain.糜蛋白酶M的结构：通过比较建模技术推导得出的晚期洗脱糜蛋白酶，以及通过pH依赖性催化动力学和与时间依赖性抑制剂反应确定的活性中心特征：半胱氨酸-25/组氨酸-159离子对对于糜蛋白酶M和木瓜蛋白酶的催化活性而言均不充分。

Biochem J. 1994 Jun 15;300 ( Pt 3)(Pt 3):805-20. doi: 10.1042/bj3000805.

Reactions of papain and of low-molecular-weight thiols with some aromatic disulphides. 2,2'-Dipyridyl disulphide as a convenient active-site titrant for papain even in the presence of other thiols.木瓜蛋白酶及低分子量硫醇与某些芳香族二硫化物的反应。2,2'-二吡啶基二硫化物作为木瓜蛋白酶便捷的活性位点滴定剂，即使在存在其他硫醇的情况下也适用。

Biochem J. 1973 May;133(1):67-80. doi: 10.1042/bj1330067.

An amplified assay for thiols based on reactivation of papain.一种基于木瓜蛋白酶重新激活的硫醇扩增检测方法。

Anal Biochem. 1993 Aug 15;213(1):49-56. doi: 10.1006/abio.1993.1384.

引用本文的文献

Preparation and characterization of a truncated caricain lacking 41 residues from the N-terminal.一种从N端缺失41个残基的截短型木瓜蛋白酶的制备与表征。

Protein J. 2005 May;24(4):243-51. doi: 10.1007/s10930-005-6720-5.

Gold-containing drugs and the control of proteolytic enzymes.含金药物与蛋白水解酶的控制

Br J Pharmacol. 1983 May;79(1):181-9. doi: 10.1111/j.1476-5381.1983.tb10511.x.

A necessary modification to the preparation of papain from any high-quality latex of Carica papaya and evidence for the structural integrity of the enzyme produced by traditional methods.对从任何优质番木瓜乳胶制备木瓜蛋白酶的必要改进以及传统方法生产的酶结构完整性的证据。

Biochem J. 1979 Feb 1;177(2):541-8. doi: 10.1042/bj1770541.

A convenient method of preparation of high-activity urease from Canavalia ensiformis by covalent chromatography and an investigation of its thiol groups with 2,2'-dipyridyl disulphide as a thiol titrant and reactivity probe.一种通过共价色谱法从刀豆中制备高活性脲酶的简便方法，以及用2,2'-二吡啶二硫化物作为硫醇滴定剂和反应性探针研究其硫醇基团。

Biochem J. 1976 Nov;159(2):245-57. doi: 10.1042/bj1590245.

Preparation of fully active ficin from Ficus glabrata by covalent chromatography and characterization of its active centre by using 2,2'-depyridyl disulphide as a reactivity probe.通过共价色谱法从光滑榕中制备完全活性的无花果蛋白酶，并使用2,2'-二吡啶基二硫化物作为反应性探针表征其活性中心。

Biochem J. 1976 Nov;159(2):221-34. doi: 10.1042/bj1590221.

A reporter group delivery system with both absolute and selective specificity for thiol groups and an improved fluorescent probe containing the 7-nitrobenzo-2-oxa-1,3-diazole moiety.一种对硫醇基团具有绝对和选择性特异性的报告基团递送系统以及一种含有7-硝基苯并-2-恶唑-1,3-二氮杂环戊二烯部分的改进型荧光探针。

Biochem J. 1975 Nov;151(2):417-32. doi: 10.1042/bj1510417.

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