Hopen G
Scand J Haematol. 1979 Mar;22(3):226-34. doi: 10.1111/j.1600-0609.1979.tb02801.x.
The influence of platelets and erythrocytes on the retention of leucocytes in glass bead columns was studied. Leucocyte retention increased when platelet retention was stimulated by the presence of erythrocytes. In the presence of only a few erythrocytes, platelet retention was low, and platelets did not influence leucocyte retention. When only a small number of platelets was present, erythrocytes inhibited leucocyte retention. The platelet-dependent leucocyte retention increased as the volume passed through the columns was increased. However, the platelet-independent leucocyte retention did not change when the sample size was increased. In blood from patient with thrombocytopenia or defect in platelet function and in normal blood treated with inhibitor of platelet adhesion, neutrophil retention was reduced. However, erythrocyte-free leucocyte suspensions from these cases showed normal leucocyte retention. Thus, experiments with whole blood may serve as a model for the study of platelet-leucocyte interaction. However, for the study of leucocyte adhesiveness per se, isolated leucocytes seem more suitable than whole blood.
研究了血小板和红细胞对玻璃珠柱中白细胞滞留的影响。当红细胞的存在刺激血小板滞留时，白细胞滞留增加。在仅存在少量红细胞的情况下，血小板滞留率较低，且血小板不影响白细胞滞留。当仅存在少量血小板时，红细胞抑制白细胞滞留。随着通过柱体的体积增加，依赖血小板的白细胞滞留增加。然而，当样本量增加时，不依赖血小板的白细胞滞留没有变化。在血小板减少症患者或血小板功能缺陷患者的血液以及用血小板黏附抑制剂处理的正常血液中，中性粒细胞滞留减少。然而，这些病例的无红细胞白细胞悬液显示出正常的白细胞滞留。因此，全血实验可作为研究血小板-白细胞相互作用的模型。然而，对于白细胞黏附性本身的研究，分离的白细胞似乎比全血更合适。
