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玻璃珠柱保留法作为白细胞黏附性的一种测量方法。I. 样本量和红细胞沉降的影响。

Retention in glass bead columns as a measure of leucocyte adhesiveness. I. Influence of sample size and erythrocyte sedimentation.

作者信息

Hopen G, Schreiner A

出版信息

Scand J Haematol. 1979 Mar;22(3):219-25. doi: 10.1111/j.1600-0609.1979.tb02800.x.

DOI:10.1111/j.1600-0609.1979.tb02800.x
PMID:109909
Abstract

Erythrocyte sedimentation significantly influenced test results when leucocyte retention in glass bead columns was used as a measure of leucocyte adhesiveness. In blood with elevated ESR, this influence was especially evident. Erythrocyte sedimentation highly increased leucocyte retention in vertical columns with a downward flow, whereas in slightly tilted columns with an upward flow, the retention was reduced. The error in measurements caused by erythrocyte sedimentation could be corrected by means of the haematocrit values in samples and eluates. Correction was better for results obtained with slightly elevated than with vertical columns. The granulocyte retention rates increased with increasing sample size, whereas lymphocyte retention remained constant.

摘要

当使用玻璃珠柱中白细胞滞留作为白细胞黏附性的测量指标时,红细胞沉降显著影响测试结果。在红细胞沉降率(ESR)升高的血液中,这种影响尤为明显。红细胞沉降显著增加了垂直柱中向下流动时的白细胞滞留,而在略微倾斜的柱中向上流动时,滞留减少。红细胞沉降引起的测量误差可以通过样本和洗脱液中的血细胞比容值进行校正。对于略微升高的柱所获得的结果,校正效果优于垂直柱。粒细胞滞留率随样本量增加而增加,而淋巴细胞滞留保持不变。

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