Liu Y K, Stallard S S, Koo V, Dannaher C L
Scand J Haematol. 1979 Mar;22(3):258-62. doi: 10.1111/j.1600-0609.1979.tb02806.x.
The fraction of granulocyte-macrophage colony-forming cells (CFC) in DNA synthetic phase in blood from 25 normal adults and those in blood and bone marrow from 8 haematologically normal subjects were evaluated by in vitro culture of cells with and without prior exposure to 3H-thymidine (12.5 muCi) for 1 h at 37 degrees C. The exposure of blood cells from normal adults to 3H-thymidine resulted in 26 +/- 10% reduction in colony formation and in 14 +/- 10% reduction in cluster formation. There was no difference in the magnitude of reduction in colony formation following exposure to 3H-thymidine by cells in blood and those in bone marrow in 6 of the 8 haematologically normal subjects. These findings indicated that about one fourth of the circulating CFC in normal adults are in proliferative state and that significant difference in proliferative states between CFC in blood and those in bone marrow probably does not exist in the majority of haematologically normal subjects.
通过对细胞进行体外培养，评估了25名正常成年人血液中处于DNA合成期的粒细胞-巨噬细胞集落形成细胞（CFC）的比例，以及8名血液学正常受试者血液和骨髓中此类细胞的比例。培养时，部分细胞先于37℃下暴露于12.5微居里的3H-胸腺嘧啶1小时，部分则不进行该处理。正常成年人血细胞暴露于3H-胸腺嘧啶后，集落形成减少26±10%，簇形成减少14±10%。8名血液学正常受试者中，有6名受试者血液和骨髓中的细胞暴露于3H-胸腺嘧啶后，集落形成减少幅度无差异。这些发现表明，正常成年人循环中的CFC约四分之一处于增殖状态，并且在大多数血液学正常受试者中，血液和骨髓中的CFC增殖状态可能不存在显著差异。
