生长抑素的一级结构，一种抑制垂体生长激素分泌的下丘脑肽。
Primary structure of somatostatin, a hypothalamic peptide that inhibits the secretion of pituitary growth hormone.
作者信息
Burgus R, Ling N, Butcher M, Guillemin R
出版信息
Proc Natl Acad Sci U S A. 1973 Mar;70(3):684-8. doi: 10.1073/pnas.70.3.684.
Abstract
Somatostatin, a peptide isolated from ovine hypothalamic tissue that inhibits the release of radioimmunoassayable growth hormone in vitro from rat or human pituitary cells or in vivo in rats, has the primary structure [Formula: see text]. The structure was established by submitting the carboxymethylated peptide, the carboxymethylated tryptic digest, and the chymotryptic digest of the peptide to Edman degradation. Degradation products were analyzed by amino-acid analysis, as well as in some cases by determination of N-termini by dansylation or by determination of phenylthiohydantoins by mass spectrometry.
摘要
生长抑素是一种从绵羊下丘脑组织中分离出的肽，它在体外可抑制大鼠或人垂体细胞释放放射免疫可测定的生长激素，在体内可抑制大鼠生长激素的释放，其一级结构为[化学式：见原文]。通过对羧甲基化肽、羧甲基化胰蛋白酶消化产物和该肽的胰凝乳蛋白酶消化产物进行埃德曼降解来确定其结构。降解产物通过氨基酸分析进行分析，在某些情况下，还通过丹磺酰化法测定N末端或通过质谱法测定苯硫代乙内酰脲来进行分析。
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