酪氨酸残基在羧肽酶B作用机制中的作用：发光研究
The role of a tyrosyl residue in the mechanism of action of carboxypeptidase B: luminescence studies.
作者信息
Shaklai N, Zisapel N, Sokolovsky M
出版信息
Proc Natl Acad Sci U S A. 1973 Jul;70(7):2025-8. doi: 10.1073/pnas.70.7.2025.
Abstract
The luminescence spectra of carboxypeptidase B indicate specific differences between the zinc and apoenzyme due to the state of tyrosyl residues presumably at the active site. These differences disappear when enzyme-substrate or enzyme-inhibitor complexes are formed, suggesting that they may reflect the interaction of a tyrosyl residue in the native enzyme with the catalytically essential zinc atom. An interpretation of the role of that tyrosyl residue in the mechanism of action of carboxypeptidase B is presented.
摘要
羧肽酶B的发光光谱表明，由于活性位点处酪氨酸残基的状态，锌酶和脱辅基酶之间存在特定差异。当形成酶-底物或酶-抑制剂复合物时，这些差异消失，这表明它们可能反映了天然酶中酪氨酸残基与催化必需的锌原子之间的相互作用。本文对该酪氨酸残基在羧肽酶B作用机制中的作用进行了解释。
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本文引用的文献
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ENERGY TRANSFER IN PROTEINS.蛋白质中的能量转移
Nature. 1964 Sep 12;203:1145-7. doi: 10.1038/2031145a0.
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EFFECT OF THE PHYSICAL ENVIRONMENT ON EXCITED STATES OF AMINO ACIDS AND PROTEINS.物理环境对氨基酸和蛋白质激发态的影响。
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The kinetics of carboxypeptidase B activity.
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The tyrosyl residues at the active site of aminopeptidase M modifications by tetranitromethane.氨肽酶M活性位点的酪氨酰残基被四硝基甲烷修饰。
FEBS Lett. 1969 Aug;4(4):262-264. doi: 10.1016/0014-5793(69)80250-4.
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Effect of pH on the phosphorescence of tryptophan, tyrosine, and proteins.
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On the nature of tyrosine phosphorescence from proteins.关于蛋白质酪氨酸磷光的本质。
Biochem Biophys Res Commun. 1966 May 25;23(4):570-5. doi: 10.1016/0006-291x(66)90768-6.
7
Evidence for tyrosine at the active center of bovine carboxypeptidase B.牛羧肽酶B活性中心存在酪氨酸的证据。
J Biol Chem. 1966 Apr 10;241(7):1648-50.
8
Techniques for measuring fluorescence and phosphorescence of biological materials.
Photochem Photobiol. 1968 Dec;8(6):589-99. doi: 10.1111/j.1751-1097.1968.tb05901.x.
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Isolation and sequence of peptides at the active center of bovine carboxypeptidase B.牛羧肽酶B活性中心肽段的分离与测序
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Differences between the conformation of arsanilazotyrosine 248 of carboxypeptidase A in the crystalline state and in solution.结晶态和溶液态下羧肽酶A中248位对氨基苯砷酸酪氨酸构象的差异。
Proc Natl Acad Sci U S A. 1971 Oct;68(10):2532-5. doi: 10.1073/pnas.68.10.2532.
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