通过凝胶电泳分离新城疫病毒的信使核糖核酸
Separation of the messenger RNAs of Newcastle disease virus by gel electrophoresis.
作者信息
Collins B S, Bratt M A
出版信息
Proc Natl Acad Sci U S A. 1973 Sep;70(9):2544-8. doi: 10.1073/pnas.70.9.2544.
Abstract
We have separated the 18-22S putative messenger RNA of Newcastle disease virus into seven species ranging in molecular weight from 0.55 to 1.53 x 10(6) using sodium dodecyl sulfate-acrylamide-gel electrophoresis at relatively high concentrations of acrylamide and for a relatively long time. Studies of the number and molecular weights of the proteins and the 18-22S RNAs of the virus suggests that these RNAs are in the right molecular weight range to code for the known proteins of Newcastle disease virus. In preliminary studies using this separation technique, we have demonstrated that: (a) there is no difference between the 18-22S RNA made during a normal infection and when genome replication is blocked; and (b) there is a strain-specific difference between the RNAs of Newcastle disease virus-AV and Newcastle disease virus-HP.
摘要
我们利用十二烷基硫酸钠-聚丙烯酰胺凝胶电泳，在相对高浓度的丙烯酰胺及较长时间条件下，将新城疫病毒18 - 22S假定信使核糖核酸分离为7种，其分子量范围为0.55至1.53×10⁶ 。对该病毒蛋白质及18 - 22S核糖核酸的数量和分子量研究表明，这些核糖核酸的分子量范围适合编码新城疫病毒的已知蛋白质。在使用这种分离技术的初步研究中，我们已证明：(a)正常感染期间产生的18 - 22S核糖核酸与基因组复制受阻时产生的18 - 22S核糖核酸之间没有差异；(b)新城疫病毒-AV与新城疫病毒-HP的核糖核酸之间存在毒株特异性差异。
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本文引用的文献
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Newcastle disease virus RNA. II. Preferential synthesis of RNA complementary to parental viral RNA by chick embryo cells.新城疫病毒核糖核酸。II. 鸡胚细胞优先合成与亲本病毒核糖核酸互补的核糖核酸。
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Electrophoretic separation of viral nucleic acids on polyacrylamide gels.病毒核酸在聚丙烯酰胺凝胶上的电泳分离。
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Preliminary analysis of the requirements for fusion from within and fusion from without by Newcastle disease virus.新城疫病毒介导的体内融合和体外融合的需求初步分析
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