新城疫病毒特异性RNA：单链RNA和杂交双链体的聚丙烯酰胺凝胶分析
Newcastle disease virus-specific RNA: polyacrylamide gel analysis of single-stranded RNA and hybrid duplexes.
作者信息
Kaverin N V, Varich N L
出版信息
J Virol. 1974 Feb;13(2):253-60. doi: 10.1128/JVI.13.2.253-260.1974.
Abstract
Newcastle disease virus-specific [(3)H]uridine-labeled 18S RNA was resolved by polyacrylamide gel electrophoresis into several components with molecular weights from 450,000 to 840,000. The analysis of 35 and 24S virus-specific RNA also revealed several components in each sedimentational class. The conversion of 18S RNA into double-stranded form by hybridization with an excess of unlabeled virion RNA improved the resolution in polyacrylamide gels and revealed at least six distinct components. The same six classes of hybrid duplexes were revealed when (32)P-labeled 50S virion RNA was hybridized with an excess of 18S RNA. The applicability of polyacrylamide gel electrophoresis of hybrid duplexes to the analysis of viral genome structure is discussed.
摘要
新城疫病毒特异性的[（3）H]尿苷标记的18S RNA通过聚丙烯酰胺凝胶电泳分离为几个分子量在450,000至840,000之间的组分。对35S和24S病毒特异性RNA的分析也揭示了每个沉降类中的几个组分。通过与过量的未标记病毒粒子RNA杂交将18S RNA转化为双链形式，提高了聚丙烯酰胺凝胶中的分辨率，并揭示了至少六个不同的组分。当（32）P标记的50S病毒粒子RNA与过量的18S RNA杂交时，也揭示了相同的六类杂交双链体。讨论了杂交双链体的聚丙烯酰胺凝胶电泳在病毒基因组结构分析中的适用性。
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