在体内对HeLa细胞的蛋白质合成进行抑制后，体外刺激多肽起始。
Stimulation of polypeptide initiation in vitro after protein synthesis inhibition in vivo in HeLa cells.
作者信息
Reichman M, Penman S
出版信息
Proc Natl Acad Sci U S A. 1973 Sep;70(9):2678-82. doi: 10.1073/pnas.70.9.2678.
Abstract
Crude cytoplasmic extracts prepared from HeLa cells actively incorporate amino acids but show little initiation of new peptides (as seen by labeling of N-terminal amino acids). In contrast, extracts prepared from cells subjected to prior inhibition of protein synthesis show a significant amount of polypeptide initiation indicated by formation of peptides with radioactive N-terminal methionine. The same result was obtained whether prior inhibition occurred with cycloheximide or by starvation for an essential amino acid. Cellular response to suppression of protein synthesis appears to be mediated through production of RNA, since it is inhibited by actinomycin but appears in the presence of cycloheximide. The crude extracts continue initiating new polypeptides for at least 10 min in vitro. It is postulated that enhancement of in vitro initiation described here is related to the apparent stimulation of initiation of translation seen in vivo.
摘要
从HeLa细胞制备的粗细胞质提取物能积极地掺入氨基酸，但新肽起始合成很少（通过N端氨基酸标记可见）。相比之下，从先前受到蛋白质合成抑制的细胞制备的提取物显示出大量的多肽起始合成，这通过具有放射性N端甲硫氨酸的肽的形成得以表明。无论先前的抑制是用环己酰亚胺还是通过必需氨基酸饥饿处理，都得到相同的结果。细胞对蛋白质合成抑制的反应似乎是通过RNA的产生介导的，因为它受到放线菌素的抑制，但在环己酰亚胺存在时出现。粗提取物在体外至少持续起始合成新多肽10分钟。据推测，这里描述的体外起始合成的增强与体内观察到的翻译起始的明显刺激有关。
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