缺乏单一必需氨基酸的HeLa细胞中蛋白质合成起始的调控
Regulation of protein synthesis initiation in HeLa cells deprived of single essential amino acids.
作者信息
Vaughan M H, Pawlowski P J, Forchhammer J
出版信息
Proc Natl Acad Sci U S A. 1971 Sep;68(9):2057-61. doi: 10.1073/pnas.68.9.2057.
Abstract
In HeLa cells deprived of valine, histidine, or methionine initiation of protein synthesis decreases rapidly and disaggregation of polyribosomes also occurs. The mechanism of inhibition does not seem to involve the supply of RNA in the cell, and thus it differs from the initiation of inhibition at elevated temperatures. Polyribosomes rapidly form again if the missing amino acid is restored, even in the presence of actinomycin D.
摘要
在缺乏缬氨酸、组氨酸或蛋氨酸的海拉细胞中，蛋白质合成的起始迅速减少，同时多核糖体也会发生解聚。抑制机制似乎并不涉及细胞内RNA的供应，因此它与高温下抑制起始的情况不同。即使存在放线菌素D，如果恢复缺失的氨基酸，多核糖体也会迅速重新形成。
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