密旋霉素对脊髓灰质炎病毒蛋白合成的影响：一种基因定位方法。
Effect of pactamycin on synthesis of poliovirus proteins: a method for genetic mapping.
作者信息
Taber R, Rekosh D, Baltimore D
出版信息
J Virol. 1971 Oct;8(4):395-401. doi: 10.1128/JVI.8.4.395-401.1971.
Abstract
We have studied the effect of the drug pactamycin on protein synthesis in poliovirus-infected HeLa cells. At a concentration which primarily inhibits initiation of protein synthesis, the spectrum of poliovirus proteins synthesized is markedly changed. The amount of NCVP 1, the capsid precursor, is greatly reduced relative to NCVP 2 and the amount of NCVP X is slightly reduced. Since it is believed that there is only one major site for the initiation of protein synthesis on the poliovirus genome, we interpret this differential effect on the poliovirus proteins to be an indication of their relative distance from the initiation site. On this basis, we propose a gene order for the poliovirus genome (5' --> 3') of NCVP 1, NCVP X, NCVP 2.
摘要
我们研究了药物巴龙霉素对脊髓灰质炎病毒感染的HeLa细胞中蛋白质合成的影响。在主要抑制蛋白质合成起始的浓度下，合成的脊髓灰质炎病毒蛋白谱发生了显著变化。相对于NCVP 2，衣壳前体NCVP 1的量大幅减少，而NCVP X的量略有减少。由于人们认为脊髓灰质炎病毒基因组上只有一个主要的蛋白质合成起始位点，我们将这种对脊髓灰质炎病毒蛋白的差异影响解释为它们与起始位点相对距离的指示。在此基础上，我们提出了脊髓灰质炎病毒基因组（5'→3'）的基因顺序为NCVP 1、NCVP X、NCVP 2。
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