显微外科融合的人类体细胞杂种：分析与克隆

Proc Natl Acad Sci U S A. 1973 Dec;70(12):3382-6. doi: 10.1073/pnas.70.12.3382.

Cell fusion by microsurgery permits production of specified bicellular hybrid cells with very high efficiency. Systematic analysis of the fate of bicellular hybrids reveals fundamental properties of cells in accordance with cell type. Bicellular hybrids that do not proliferate survive 1-21 days; those that do proliferate form clones 8-9 days after fusion.

通过显微手术进行细胞融合能够高效地产生特定的双细胞杂交细胞。对双细胞杂交细胞命运的系统分析揭示了根据细胞类型而定的细胞基本特性。不增殖的双细胞杂交细胞可存活1至21天；那些增殖的细胞在融合后8至9天形成克隆。