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通过显微手术实现哺乳动物体细胞融合。

Fusion of mammalian somatic cells by microsurgery.

作者信息

Diacumakos E G, Tatum E L

出版信息

Proc Natl Acad Sci U S A. 1972 Oct;69(10):2959-62. doi: 10.1073/pnas.69.10.2959.

DOI:10.1073/pnas.69.10.2959
PMID:4507616
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC389683/
Abstract

Mammalian somatic-cell hybrids can be formed in a direct and precise manner by microsurgery. Fusion of cells brought about in this way permits selection of specific, identifiable cells of the same or different types or species and their fusion at telophase.

摘要

哺乳动物体细胞杂种可以通过显微手术直接精确地形成。以这种方式实现的细胞融合允许选择相同或不同类型或物种的特定、可识别的细胞，并在末期使其融合。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/34eb/389683/0ed36ea44ad5/pnas00088-0211-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/34eb/389683/462fcc06d320/pnas00088-0210-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/34eb/389683/0ed36ea44ad5/pnas00088-0211-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/34eb/389683/462fcc06d320/pnas00088-0210-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/34eb/389683/0ed36ea44ad5/pnas00088-0211-a.jpg

相似文献

1
Fusion of mammalian somatic cells by microsurgery.通过显微手术实现哺乳动物体细胞融合。
Proc Natl Acad Sci U S A. 1972 Oct;69(10):2959-62. doi: 10.1073/pnas.69.10.2959.
2
Microsurgically fused human somatic cell hybrids: analysis and cloning.显微外科融合的人类体细胞杂种：分析与克隆
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Limited lifespan in somatic cell hybrids and cybrids.体细胞杂种和胞质杂种的寿命有限。
Exp Cell Res. 1980 Jun;127(2):385-96. doi: 10.1016/0014-4827(80)90443-7.
4
Methods for microsurgical production of mammalian somatic cell hybrids and their analysis and cloning.
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5
Methods for micromanipulation of human somatic cells in culture.培养人类体细胞的显微操作方法。
Methods Cell Biol. 1973;7:287-311. doi: 10.1016/s0091-679x(08)61783-5.
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Isolation and analysis of somatic hybrids derived from two human diploid cells.源自两个人类二倍体细胞的体细胞杂种的分离与分析。
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The membrane junctions in communicating and noncommunicating cells, their hybrids, and segregants.
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Sendai produced somatic cell hybrids between L cell strains and between liver and L cells.仙台病毒诱导了L细胞株之间以及肝脏细胞与L细胞之间的体细胞杂交。
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Presence of two active X chromosomes in hybrids between normal human and SV40-transformed fibroblasts from patients with the Lesch-Nyhan syndrome.在正常人与患有莱施-奈恩综合征患者的SV40转化成纤维细胞的杂交细胞中存在两条活跃的X染色体。
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10
Karyological properties of human-mouse somatic hybrids.人-鼠体细胞杂种的核型特征
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Chromosome mapping of connective tissue protein genes.结缔组织蛋白基因的染色体定位
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Microsurgically fused human somatic cell hybrids: analysis and cloning.显微外科融合的人类体细胞杂种：分析与克隆

本文引用的文献

1
A microsurgical methodology for human cells in vitro: evolution and applications.一种用于体外培养人类细胞的显微外科方法：演变与应用
Proc Natl Acad Sci U S A. 1970 Apr;65(4):911-8. doi: 10.1073/pnas.65.4.911.
2
Cell fusion without viruses.无病毒的细胞融合
Biochem J. 1971 Oct;124(5):46P-47P. doi: 10.1042/bj1240046p.
3
Chromosome displacement in and extraction from human cells at different mitotic stages.
Nature. 1971 Jul 2;232(5305):33-6. doi: 10.1038/232033a0.
Proc Natl Acad Sci U S A. 1973 Dec;70(12):3382-6. doi: 10.1073/pnas.70.12.3382.
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Utilization of somatic cell hybrids for genetic studies in man.利用体细胞杂种进行人类遗传学研究。
Humangenetik. 1973;19(1):1-40. doi: 10.1007/BF00295233.
5
The fusion of erythrocytes by fatty acids, esters, retinol and alpha-tocopherol.红细胞与脂肪酸、酯类、视黄醇和α-生育酚的融合。
Biochem J. 1973 Sep;136(1):147-55. doi: 10.1042/bj1360147.
6
Cell fusion and some subcellular properties of heterokaryons and hybrids.细胞融合以及异核体和杂种细胞的一些亚细胞特性。
J Cell Biol. 1975 Nov;67(2PT.1):257-80. doi: 10.1083/jcb.67.2.257.
7
Membrane events in the acrosomal reaction of Limulus sperm. Membrane fusion, filament-membrane particle attachment, and the source and formation of new membrane surface.鲎精子顶体反应中的膜事件。膜融合、细丝-膜颗粒附着以及新膜表面的来源和形成。
J Cell Biol. 1979 Apr;81(1):229-53. doi: 10.1083/jcb.81.1.229.
4
Microsurgical studies on human cells and cloning of HeLa cells.关于人类细胞的显微外科研究及海拉细胞的克隆
Nature. 1971 Jul 2;232(5305):28-32. doi: 10.1038/232028a0.
5
Effect of environmental pH on the efficiency of cellular hybridization.环境pH对细胞杂交效率的影响。
Proc Natl Acad Sci U S A. 1972 Jul;69(7):1953-6. doi: 10.1073/pnas.69.7.1953.
6
Induction of homokaryocyte, heterokaryocyte and hybrid formation by lysolecithin.溶血卵磷脂诱导同核体细胞、异核体细胞及杂种形成
Exp Cell Res. 1971 Aug;67(2):427-35. doi: 10.1016/0014-4827(71)90428-9.
7
Fusion of cells by HVJ: requirement of concentration of virus particles at the site of contact of two cells for fusion.
Exp Cell Res. 1968 Sep;52(1):34-42. doi: 10.1016/0014-4827(68)90545-4.
8
Poliovirus replication and cytopathogenicity in monolayer hamster cell cultures fused with beta propiolactone-inactivated Sendai virus.脊髓灰质炎病毒在与β-丙内酯灭活仙台病毒融合的单层仓鼠细胞培养物中的复制及细胞致病性
Proc Soc Exp Biol Med. 1968 Jan;127(1):260-7. doi: 10.3181/00379727-127-32668.