在一项针对起始因子的特定测试中使用来自兔网织红细胞的纯化多核糖体。
Use of purified polysomes from rabbit reticulocytes in a specific test for initiation factors.
作者信息
Lubsen N H, Davis B D
出版信息
Proc Natl Acad Sci U S A. 1974 Jan;71(1):68-72. doi: 10.1073/pnas.71.1.68.
Initiation factors, extracted from ribosomal particles by high salt, are usually tested for with the extracted ribosomes. However, it is theoretically possible that such tests may respond not only to factors (i.e., proteins that join and leave a ribosome during its cycle), but also to true ribosomal proteins that may have been extracted. We have eliminated this circularity by tests with purified polysomes, which have not been extracted with high salt; the polysomes are deficient in initiation factors because they have been largely separated from the native subunits. Tests with both systems, applied to extracts of different classes of particles, suggest that high salt does extract both initiation factors and essential ribosomal proteins. Extracts of native subunits contain both, and stimulate both systems; while, in the presence of these extracts, the extracts of polysomes further stimulate only the extracted ribosomes.
通过高盐从核糖体颗粒中提取的起始因子，通常会与提取出的核糖体一起进行检测。然而，从理论上来说，这样的检测可能不仅会对起始因子（即在核糖体循环过程中与核糖体结合并离开的蛋白质）产生反应，还可能对可能已被提取的真正核糖体蛋白产生反应。我们通过使用未用高盐提取的纯化多聚核糖体进行检测，消除了这种循环性；这些多聚核糖体缺乏起始因子，因为它们已与天然亚基大量分离。对不同类型颗粒提取物应用这两种系统进行的检测表明，高盐确实会提取起始因子和必需的核糖体蛋白。天然亚基的提取物同时含有这两者，并能刺激这两种系统；而在这些提取物存在的情况下，多聚核糖体的提取物仅能进一步刺激提取出的核糖体。
Zotero 插件