抑制镰状血红蛋白凝胶化的化学修饰。
Chemical modifications that inhibit gelation of sickle hemoglobin.
作者信息
Benesch R, Benesch R E, Yung S
出版信息
Proc Natl Acad Sci U S A. 1974 Apr;71(4):1504-5. doi: 10.1073/pnas.71.4.1504.
Abstract
Substitution of the N-terminal amino groups with pyridoxal compounds inhibits gelation and increases the solubility of deoxy sickle hemoglobin (Hb S). Pyridoxylation of the alpha chains has considerably more effect than that of the beta chains. The increase in minimum gelling concentration of Hb S that results from modification of the alpha N-termini is the same as that produced by dilution of Hb S with an equal amount of Hb A.
摘要
用吡哆醛化合物取代N端氨基可抑制凝胶化并增加脱氧镰状血红蛋白（Hb S）的溶解度。α链的吡哆醛化作用比β链的大得多。由α N端修饰导致的Hb S最低凝胶化浓度的增加与用等量Hb A稀释Hb S所产生的增加相同。
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本文引用的文献
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Sickle cell anemia a molecular disease.镰状细胞贫血是一种分子疾病。
Science. 1949 Nov 25;110(2865):543-8. doi: 10.1126/science.110.2865.543.
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X-ray and solubility studies of the haemoglobin of sickle-cell anaemia patients.镰状细胞贫血患者血红蛋白的X射线和溶解性研究。
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Gene mutations in human haemoglobin: the chemical difference between normal and sickle cell haemoglobin.人类血红蛋白中的基因突变：正常血红蛋白与镰状细胞血红蛋白的化学差异。
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Reduction of methaemoglobin in haemoglobin samples using gel filtration for continuous removal of reaction products.使用凝胶过滤法降低血红蛋白样本中的高铁血红蛋白，以持续去除反应产物。
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