镰状细胞血红蛋白的预凝胶化聚集

Pregelation aggregation of sickle cell hemoglobin.

作者信息

Wilson W W, Luzzana M R, Penniston J T, Johnson C S

出版信息

Proc Natl Acad Sci U S A. 1974 Apr;71(4):1260-3. doi: 10.1073/pnas.71.4.1260.

DOI:10.1073/pnas.71.4.1260

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC388205/

Abstract

Quasi-elastic light scattering was used to detect the initial stage of aggregation of hemoglobin S solutions. Aggregation of a hemolysate upon deoxygenation was found to occur at concentrations above 11 g/dl, but purified hemoglobin S solutions showed evidence of aggregation only for concentrations greater than 14 g/dl. A transition from a nonaggregated to an aggregated state was observed when the oxygen pressure in solution was reduced below a critical value. The total intensity of the scattered light during the early stages of aggregation was found to increase linearly with time. This result is consistent with a linear association process in which any size aggregate can combine with any other size aggregate.

摘要

准弹性光散射被用于检测血红蛋白S溶液聚集的初始阶段。发现当浓度高于11 g/dl时，溶血产物在脱氧时会发生聚集，但纯化的血红蛋白S溶液仅在浓度大于14 g/dl时显示出聚集迹象。当溶液中的氧压降低到临界值以下时，观察到从非聚集状态到聚集状态的转变。发现在聚集早期散射光的总强度随时间线性增加。该结果与线性缔合过程一致，在该过程中任何大小的聚集体都可以与任何其他大小的聚集体结合。

相似文献

1

Pregelation aggregation of sickle cell hemoglobin.镰状细胞血红蛋白的预凝胶化聚集

Proc Natl Acad Sci U S A. 1974 Apr;71(4):1260-3. doi: 10.1073/pnas.71.4.1260.

2

Studies on the mechanism of action of cyanate in sickle cell disease. Oxygen affinity and gelling properties of hemoglobin S carbamylated on specific chains.镰状细胞病中氰酸盐作用机制的研究。特定链上氨基甲酰化血红蛋白S的氧亲和力和凝胶化特性。

J Biol Chem. 1974 Oct 25;249(20):6611-6.

3

Conformational requirements for the polymerization of hemoglobin S: studies of mixed liganded hybrids.

J Mol Biol. 1973 May 15;76(2):233-9. doi: 10.1016/0022-2836(73)90387-2.

4

A temperature-dependent latent-period in the aggregation of sickle-cell deoxyhemoglobin.

Biochem Biophys Res Commun. 1974 Aug 5;59(3):887-93. doi: 10.1016/s0006-291x(74)80062-8.

5

Chemical modifications that inhibit gelation of sickle hemoglobin.抑制镰状血红蛋白凝胶化的化学修饰。

Proc Natl Acad Sci U S A. 1974 Apr;71(4):1504-5. doi: 10.1073/pnas.71.4.1504.

6

Delay time of hemoglobin S polymerization prevents most cells from sickling in vivo.血红蛋白S聚合的延迟时间可防止大多数细胞在体内发生镰变。

Science. 1987 Jul 31;237(4814):500-6. doi: 10.1126/science.3603036.

7

[Sickle cell anemia and the affinity of the blood for oxygen].[镰状细胞贫血与血液对氧气的亲和力]

C R Seances Soc Biol Fil. 1979;173(2):303-13.

8

Photon scattering as a probe of microviscosity and channel size in gels such as sickle haemoglobin.光子散射作为一种探测凝胶（如镰状血红蛋白）微观粘度和通道大小的手段。

Nature. 1983;302(5907):412-5. doi: 10.1038/302412a0.

9

Sickle hemoglobin polymerization in solution and in cells.镰状血红蛋白在溶液中和细胞中的聚合。

Annu Rev Biophys Biophys Chem. 1985;14:239-63. doi: 10.1146/annurev.bb.14.060185.001323.

10

The problem of describing gelation in vivo.描述体内凝胶化的问题。

Prog Clin Biol Res. 1987;240:237-44.

引用本文的文献

1

Diffusion coefficients of hemoglobin by intensity fluctuation spectroscopy: effects of varying pH and ionic strength.通过强度波动光谱法测定血红蛋白的扩散系数：不同pH值和离子强度的影响

Biophys J. 1981 Jan;33(1):63-79. doi: 10.1016/S0006-3495(81)84872-2.

2

Saturation transfer electron paramagnetic resonance detection of sickle hemoglobin aggregation during deoxygenation.脱氧过程中镰状血红蛋白聚集的饱和转移电子顺磁共振检测

Biophys J. 1983 Jun;42(3):269-74. doi: 10.1016/S0006-3495(83)84394-X.

3

Spin label detection of intermolecular interactions in carbonmonoxy sickle hemoglobin.一氧化碳镰状血红蛋白分子间相互作用的自旋标记检测

Biophys J. 1978 Nov;24(2):517-24. doi: 10.1016/S0006-3495(78)85398-3.

4

Intermolecular interactions of oxygenated sickle hemoglobin molecules in cells and cell-free solutions.细胞及无细胞溶液中氧化镰状血红蛋白分子的分子间相互作用

Biophys J. 1976 Jun;16(6):679-89. doi: 10.1016/S0006-3495(76)85721-9.

5

Letter: Analysis of hemoglobin aggregation from Gibbs-Donnan equilibrium experiments.信函：基于吉布斯-唐南平衡实验对血红蛋白聚集的分析

J Gen Physiol. 1976 Jul;68(1):105-9. doi: 10.1085/jgp.68.1.105.

本文引用的文献

1

The determination of the isoelectric and isoionic points of haemoglobin from measurements of membrane potentials.通过膜电位测量确定血红蛋白的等电点和等离子点。

Biochem J. 1934;28(4):1230-58. doi: 10.1042/bj0281230.

2

DETERMINATION OF OXYGEN EQUILIBRIA WITH A VERSATILE NEW TONOMETER.使用新型多功能张力计测定氧平衡

Anal Biochem. 1965 Apr;11:81-7. doi: 10.1016/0003-2697(65)90045-x.

3

Properties of sickle-cell haemoglobin.镰状细胞血红蛋白的特性。

Biochem J. 1957 Feb;65(2):212-9. doi: 10.1042/bj0650212.

4

Studies on abnormal hemoglobins. VIII. The gelling phenomenon of sickle cell hemoglobin: its biologic and diagnostic significance.异常血红蛋白的研究。VIII. 镰状细胞血红蛋白的凝胶化现象：其生物学及诊断意义。

Blood. 1953 Nov;8(11):1008-23.

5

Studies on the heterogeneity of hemoglobin. IX. The use of Tris(hydroxymethyl)aminomethanehcl buffers in the anion-exchange chromatography of hemoglobins.血红蛋白异质性的研究。IX. 三（羟甲基）氨基甲烷盐酸盐缓冲液在血红蛋白阴离子交换色谱中的应用。

J Chromatogr. 1965 Jul;19(1):160-9. doi: 10.1016/s0021-9673(01)99434-8.

6

Molecular pathology of human haemoglobin.人类血红蛋白的分子病理学

Nature. 1968 Aug 31;219(5157):902-9. doi: 10.1038/219902a0.

7

The binding of carbon dioxide by horse haemoglobin.马血红蛋白对二氧化碳的结合作用。

Biochem J. 1971 Aug;124(1):31-45. doi: 10.1042/bj1240031.

8

Ligand-induced conformational dependence of hemoglobin in sickling interactios.镰状细胞相互作用中血红蛋白的配体诱导构象依赖性

J Mol Biol. 1971 Sep 14;60(2):263-70. doi: 10.1016/0022-2836(71)90292-0.

9

The self-diffusion coefficients of myoglobin and hemoglobin in concentrated solutions.肌红蛋白和血红蛋白在浓缩溶液中的自扩散系数。

J Biol Chem. 1972 Feb 10;247(3):895-901.

10

Sickle hemoglobin gelation--inhibition by tris (hydroxymethyl) aminomethane and sugars.镰状血红蛋白凝胶化——三（羟甲基）氨基甲烷和糖类的抑制作用

Biochem Pharmacol. 1973 Mar 15;22(6):667-74. doi: 10.1016/0006-2952(73)90399-7.

文献AI研究员

20分钟写一篇综述，助力文献阅读效率提升50倍。

用中文搜PubMed

大模型驱动的PubMed中文搜索引擎

文档翻译

学术文献翻译模型，支持多种主流文档格式。