Gospodarowicz D, Jones K L, Sato G
Proc Natl Acad Sci U S A. 1974 Jun;71(6):2295-9. doi: 10.1073/pnas.71.6.2295.
The purification of a growth factor from bovine pituitary glands that controls the division of an ovarian cell line maintained in tissue culture is reported. Extraction of bovine pituitary glands at pH 4.5 followed by ammonium sulfate precipitation, chromatography on carboxymethylcellulose, and gel filtration on Sephadex G-50 gives yields of ovarian growth factor of about 7 mg/kg of glands. Contamination by other pituitary hormones was not detected. The preparation obtained has been characterized with respect to biological activity, molecular weight, and amino-acid composition. The implication of this growth factor in the development of ovarian tumors is discussed.
据报道，已从牛垂体中纯化出一种生长因子，该因子可控制在组织培养中维持的一种卵巢细胞系的分裂。在pH 4.5条件下提取牛垂体，随后进行硫酸铵沉淀、羧甲基纤维素柱色谱和葡聚糖G - 50凝胶过滤，卵巢生长因子的产量约为每千克腺体7毫克。未检测到其他垂体激素的污染。所获得的制剂已在生物活性、分子量和氨基酸组成方面进行了表征。本文还讨论了这种生长因子在卵巢肿瘤发生发展中的意义。
