通过胰蛋白酶处理增强无细胞哺乳动物系统中的DNA合成。
Enhancement of DNA synthesis in a mammalian cell-free system by trypsin treatment.
作者信息
Brown R L, Stubblefield E
出版信息
Proc Natl Acad Sci U S A. 1974 Jun;71(6):2432-4. doi: 10.1073/pnas.71.6.2432.
Abstract
DNA synthesis in a broken cellular preparation of Chinese hamster cells was enhanced approximately 10-fold by a brief trypsin treatment. Alphachymotrypsin also enhanced the synthesis, whereas Pronase did not. The trypsin appears to be acting on a component of the nucleus. Evidence suggests that the trypsin is not removing protein from the DNA, but may be activating the system some other way.
摘要
用胰蛋白酶短暂处理中国仓鼠细胞破碎后的细胞制剂，可使DNA合成增强约10倍。α-糜蛋白酶也能增强合成，而链霉蛋白酶则不能。胰蛋白酶似乎作用于细胞核的一种成分。有证据表明，胰蛋白酶并非从DNA上去除蛋白质，而是可能以其他方式激活该系统。
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