Duplan J F, Legrand E, Castaignos C, de Calignon E
Int J Radiat Biol Relat Stud Phys Chem Med. 1979 Dec;36(6):595-600. doi: 10.1080/09553007914551421.
Experiments were carried out to compare the therapeutic efficiency (TE: number of CFU required to reduce the mortality from 100 to 50 per cent) of spleen or marrow (BM) stem cells (CFU) grafted into lethally irradiated mice (807 rad) which had been previously treated with 89Sr or splenectomized. It was found that during the reconstitution of the haemopoietic organs, the spleen does not provide more than 10 per cent of the functional cells necessary for survival. Besides, the BM-derived CFU growing in 89Sr marrow-ablated mice remain twice as efficient as the spleen-derived ones. Similarly, spleen-derived CFU transplanted into splenectomized mice are half as efficient as BM-derived ones. It may therefore be assumed that haemopoietic stem cells grafted into a foreign microenvironment retain their original kinetics of growth and differentiation during 7 to 10 days after their transplantation.
进行了实验，以比较移植到经89Sr预处理或脾切除的受致死剂量照射（807拉德）小鼠体内的脾或骨髓（BM）干细胞（CFU）的治疗效率（TE：使死亡率从100%降至50%所需的CFU数量）。结果发现，在造血器官的重建过程中，脾脏提供的存活所需功能细胞不超过10%。此外，在89Sr骨髓消融小鼠中生长的BM来源的CFU的效率仍然是脾脏来源CFU的两倍。同样，移植到脾切除小鼠体内的脾脏来源的CFU的效率是BM来源CFU的一半。因此可以假定，移植到异体微环境中的造血干细胞在移植后7至10天内保持其原始的生长和分化动力学。
